Platelet derived growth factor and fibroblast growth factor basic levels in the vitreous of patients with vitreoretinal disorders
Platelet derived growth factor and fibroblast growth factor basic levels in the vitreous of patients with vitreoretinal disorders
复制标题
DOI：
10.1136/bjo.82.2.181
复制
发表时间：
1998-02-01
影响因子：
4.1
通讯作者：
Kennedy, S
中科院分区：
文献类型：
--
作者：
Cassidy, L;Barry, P;Kennedy, S
目的：探讨碱性成纤维细胞生长因子（bFGF）和血小板衍生生长因子（PDGF）在增殖性玻璃体视网膜病变（PVR）发病机制中的潜在作用。方法：采用酶联免疫吸附法测定38例玻璃体标本中bFGF和PDGF的含量，这些玻璃体标本来自于因各种玻璃体视网膜疾病而行经平面肌玻璃体切除术的患者。结果：bfgf水平从检测不到到318.7 pg/ml不等。平均浓度为27.57 pg/ml。PDGF水平从检测不到到160 pg/ml，平均浓度为40.06 pg/ml。13例有视网膜脱离（RD）和PVR临床证据的患者中有8例bFGF浓度显著升高，而11例有RD和无PVR的患者中只有1例可检测到bFGF；8例RD和PVR患者中有7例玻璃体PDGF浓度升高，而10例RD和无PVR患者玻璃体PDGF均未检测到。8例玻璃体出血患者PDGF浓度升高，其中2例新生血管活跃患者PDGF浓度特别高（120 pg/ml）。9例玻璃体出血患者中有2例bFGF水平升高。结论：即使在没有玻璃体出血的PVR患者中，bfgf和EDGE浓度也会升高，而在未合并PVR的RD患者中则不会升高。这一观察结果表明，这两种细胞因子可能参与PVR的发病机制。
Aim-To determine the potential role of basic fibroblast growth factor (bFGF) and platelet derived growth factor (PDGF) in the pathogenesis of proliferative vitreoretinopathy (PVR).Methods-An enzyme Linked immunosorbent assay technique was used to determine the quantities of bFGF and PDGF in 38 vitreous samples taken from patients undergoing trans pars plana vitrectomy (PPV) for a variety of vitreoretinal disorders.Results-bFGF levels ranged from undetectable to 318.7 pg/ml. The mean concentration was 27.57 pg/ml. PDGF levels ranged from undetectable to 160 pg/ml, the mean concentration being 40.06 pg/ml. Eight of 13 patients with clinical evidence of retinal detachment (RD) and PVR had significantly elevated bFGF concentrations, whereas only one of 11 patients with RD and no PVR had detectable bFGF; seven of eight patients with RD and PVR had elevated PDGF concentrations in the vitreous, whereas all 10 patients with RD and no PVR had no detectable vitreous PDGF. Eight patients with vitreous haemorrhage had raised PDGF concentrations, and the levels were particularly high (>120 pg/ml) in those two patients with active neovascularisation. Two out of nine patients with vitreous haemorrhage had raised bFGF levels.Conclusions-bFGF and EDGE concentrations are elevated in PVR even in the absence of vitreous haemorrhage, and not in patients with RD uncomplicated by PVR. This observation suggests that both cytokines may be involved in the pathogenesis of PVR.