Effects of ghrelin on developmental competence and gene expression of in vitro fertilized ovine embryos
Effects of ghrelin on developmental competence and gene expression of in vitro fertilized ovine embryos
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ghrelin对体外受精绵羊胚胎发育能力和基因表达的影响
DOI：
10.1016/j.theriogenology.2012.11.026
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发表时间：
2013-03-01
期刊：
影响因子：
2.8
通讯作者：
Yu, Songdong
中科院分区：
文献类型：
--
作者：
Wang, Zhengguang;Lin, Ping;Yu, Songdong
目的是确定体外卵母细胞成熟（IVM）和体外胚胎培养（IVC）期间不同生长素浓度（0、10、50和250 ng/mL）对绵羊胚胎发育和参与转录调节（OCT 4）的基因表达模式的影响葡萄糖转运（GLUT 1）、妊娠识别（IFNT）和生长素受体（GHSR-1a）。250 ng/mLGhrelin组卵母细胞的卵裂率和囊胚形成率显著低于0、10和50 ng/mLGhrelin组（P < 0.05）。与0和10 ng/mL ghrelin相比，在IVC期间或在IVM和IVC期间添加50 ng/mL ghrelin显著增加囊胚率（分别为35.3%和36.7% vs. 26.9%和26.8%）和每个囊胚的总细胞数（110.4和108.3 vs. 81.2和77.4）。但高浓度（250 ng/mL）的ghrelin在体外成熟和体外循环中的卵裂率、囊胚率和囊胚细胞总数均低于低浓度（P < 0.05）。50 ng/mL Ghrelin处理组囊胚内GLUT 1和IFNT转录本的相对丰度显著高于其他浓度组（P < 0.05）。与其他处理相比，在IVM期间加入10 ng/mL ghrelin（0.079）或在IVM和IVC期间加入10 ng/mL ghrelin（0.053）时GHSR-1a的表达更高。然而，添加较高浓度（50或250 ng/mL）的ghrelin降低了GHSR-1a转录本的相对丰度（0.032和0.039，P < 0.05）。总之，适当浓度的生长激素释放肽促进绵羊囊胚形成在体外和GLUT 1，IFNT，和GHSR-1a基因的表达增加囊胚，虽然高浓度的生长激素释放肽抑制胚胎发育。(c)2013 Elsevier Inc. All rights reserved.
The objective was to determine the effects of various ghrelin concentrations (0, 10, 50, and 250 ng/mL) during in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on ovine embryo development and expression patterns of genes involved in transcription regulation (OCT4), glucose transport (GLUT1), pregnancy recognition (IFNT), and ghrelin receptor (GHSR-1a). Rates of cleavage and blastocyst formation were decreased when oocytes were matured with 250 ng/mL ghrelin compared with 0, 10, or 50 ng/mL ghrelin (P < 0.05). Addition of 50 ng/mL ghrelin during IVC or during IVM and IVC significantly increased blastocyst rates (35.3% and 36.7% vs. 26.9% and 26.8%, respectively) and total cell numbers per blastocyst (110.4 and 108.3 vs. 81.2 and 77.4) compared with 0 and 10 ng/mL ghrelin. However, a high concentration (250 ng/mL) of ghrelin during IVM and IVC decreased cleavage, blastocyst rate, and total cell number of blastocyst compared with low concentrations (P < 0.05). Relative abundances of GLUT1 and IFNT transcripts were higher in blastocysts treated with 50 ng/mL ghrelin during IVC compared with other concentrations (P < 0.05). Expression of GHSR-1a was higher when 10 ng/mL ghrelin was added during IVM (0.079) or during IVM and IVC (0.053) compared with other treatments. However, addition of ghrelin at higher concentrations (50 or 250 ng/mL) reduced relative abundances of GHSR-1a transcripts (0.032 and 0.039, P < 0.05). In conclusion, appropriate concentrations of ghrelin promoted ovine blastocyst formation in vitro and increased expression of GLUT1, IFNT, and GHSR-1a genes in blastocysts, although a high concentration of ghrelin suppressed embryo development. (c) 2013 Elsevier Inc. All rights reserved.