Preparation of biodiesel by lipase-catalyzed transesterification of high free fatty acid containing oil from Madhuca indica
Preparation of biodiesel by lipase-catalyzed transesterification of high free fatty acid containing oil from Madhuca indica
复制标题
DOI：
10.1021/ef0602168
复制
发表时间：
2007-01-01
期刊：
影响因子：
5.3
通讯作者：
Gupta, Munishwar N.
中科院分区：
文献类型：
--
作者：
Kumari, Veena;Shah, Shweta;Gupta, Munishwar N.
蓖麻油中游离脂肪酸含量高，因此很难用化学催化剂将其转化为生物柴油。在这项工作中，以洋葱假单胞菌为原料制备脂肪酶作为催化剂，将洋葱假单胞菌油转化为乙酯。该工艺采用无溶剂介质。工艺优化包括不同的参数，如反应介质中的水分含量和在脂肪酶用作粉末之前干燥过程中存在的盐。固定化的脂肪酶在6小时内转化率为96%。使用交联酶聚集体（CLEAs）和蛋白包被微晶体（PCMCs）改性生物催化剂的效果最好。工艺优化后的游离酶粉在50 mg脂肪酶的作用下，6 h转化率为98%，CLEAs在2.5 h（相当于50 mg酶）转化率为92%，PCMCs在2.5 h（相同量的酶）转化率为99%。在pcmc的情况下，99%的转化率可以通过使用相当于25mg酶的生物催化剂获得，但需要延长16h的时间。
The oil from Madhuca indica has a high free fatty acid content, and hence, it is difficult to convert it into biodiesel by using a chemical catalyst. In this work, a commercial preparation of lipase from Pseudomonas cepacia was used as a catalyst for converting this oil into ethyl esters. Solvent-free media were used for this process. The process optimization consisted of varying parameters, such as water content in the reaction medium and salt present during the drying of lipase prior to its use as powder. The lipase immobilized on accurel gave 96% conversion in 6 h. The best results were obtained using modified biocatalyst formulations, which are called cross-linked enzyme aggregates (CLEAs) and protein-coated microcrystals (PCMCs). While free enzyme powder after process optimization gave 98% conversion in 6 h using 50 mg of lipase, CLEAs gave 92% conversion in 2.5 h (using an equivalent of 50 mg of enzyme) and PCMCs gave 99% conversion in 2.5 h using the same amount of enzyme. In the case of PCMCs, 99% conversion could be obtained just by using a biocatalyst equivalent to 25 mg of enzyme but with an extended time period of 16 h.