Broadly tuned and respiration-independent inhibition in the olfactory bulb of awake mice
Broadly tuned and respiration-independent inhibition in the olfactory bulb of awake mice
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DOI：
10.1038/nn.3669
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发表时间：
2014-04-01
影响因子：
25
通讯作者：
Shea, Stephen D.
中科院分区：
文献类型：
--
作者：
Cazakoff, Brittany N.;Lau, Billy Y. B.;Shea, Stephen D.
嗅觉表征是由大脑状态和呼吸形成的。这些影响的相互作用和电路基质尚不清楚。据推测，主嗅球（MOB）中的颗粒细胞（GCs）通过抑制二尖瓣/簇状细胞（MTs）来塑造到达皮层的活动。gc可能通过促进mt之间的侧向抑制和/或强制暂时锁定呼吸的精确活动，使集合活动更加稀疏。然而，清醒GC活动的选择性和时间结构尚不清楚。我们在麻醉和清醒小鼠的MOB中记录了GCs，并确定了气味编码和时间模式的状态依赖性特征。麻醉下，GCs稀疏活跃，与呼吸强烈同步耦合。醒来后，gc失去了同步，扩大了它们的调谐，并在很大程度上独立于呼吸。因此，在清醒期间，GCs表现出更强的气味反应，而时间结构较少。我们提出，在清醒期间，GCs可能通过扩大的横向相互作用而不是呼吸同步来塑造MT气味反应。
Olfactory representations are shaped by brain state and respiration. The interaction and circuit substrates of these influences are unclear. Granule cells (GCs) in the main olfactory bulb (MOB) are presumed to sculpt activity reaching the cortex via inhibition of mitral/tufted cells (MTs). GCs potentially make ensemble activity more sparse by facilitating lateral inhibition among MTs and/or enforce temporally precise activity locked to breathing. Yet the selectivity and temporal structure of wakeful GC activity are unknown. We recorded GCs in the MOB of anesthetized and awake mice and identified state-dependent features of odor coding and temporal patterning. Under anesthesia, GCs were sparsely active and strongly and synchronously coupled to respiration. Upon waking, GCs desynchronized, broadened their tuning and largely fired independently from respiration. Thus, during wakefulness, GCs exhibited stronger odor responses with less temporal structure. We propose that during wakefulness GCs may shape MT odor responses through broadened lateral interactions rather than respiratory synchronization.