Assessment of the cytotoxicity of photocrosslinked dextran and hyaluronan-based hydrogels to vascular smooth muscle cells.
Assessment of the cytotoxicity of photocrosslinked dextran and hyaluronan-based hydrogels to vascular smooth muscle cells.
复制标题
DOI：
10.1016/s0142-9612(02)00009-1
复制
发表时间：
2002-08
期刊：
影响因子：
14
通讯作者：
J. Trudel;S. Massia
中科院分区：
文献类型：
--
作者：
J. Trudel;S. Massia
在将材料与血管平滑肌细胞间接接触48小时后，在体外研究了基于多糖的水凝胶和溶液的细胞毒性。使用甲基丙烯酸缩水甘油酯衍生葡聚糖和/或透明质酸，并在多孔插入物中在光引发剂和紫外线辐射的存在下形成水凝胶，以避免与细胞单层直接接触。细胞形态学观察表明，葡聚糖水凝胶，非衍生化透明质酸到葡聚糖水凝胶的共混物，和透明质酸溶液是高度细胞相容的。然而，与含有多孔插入物但不含水凝胶的未暴露假对照相比，由衍生透明质酸制成的水凝胶具有细胞毒性。增殖和活力的定量测定结果证实了定性观察结果，刮伤试验显示间接暴露于几种多糖基材料后平滑肌细胞迁移/增殖显著增加。本研究的结果表明，由葡聚糖和透明质酸溶液制成的水凝胶在体外显示出良好的细胞相容性，使得这些可降解基质成为药物递送目的的有趣候选物。
The cytotoxicity of polysaccharide-based hydrogels and solutions was studied in vitro after 48h of indirect exposure of the materials with vascular smooth muscle cells. Dextran and/or hyaluronan were derivatized using glycidyl methacrylate, and hydrogels were formed in the presence of photoinitiators and ultraviolet radiation in multiwell inserts to avoid direct contact with cell monolayers. Observation of cell morphology indicated that dextran hydrogels, a blend of non-derivatized hyaluronan into dextran hydrogel, and a hyaluronan solution were highly cytocompatible. However, hydrogels made of derivatized hyaluronan were cytotoxic when compared to unexposed sham controls that contained multiwell inserts but no hydrogels. Results from quantitative assays for proliferation and viability corroborated the qualitative observations, and scrape wound assays revealed a significant increase in smooth muscles cell migration/proliferation after indirect exposure to several of the polysaccharide-based materials. Results from this study demonstrate that hydrogels made of dextran and hyaluronan solution show good cytocompatibility in vitro, making these degradable matrices interesting candidates for drug delivery purposes.