Type 2 lysophosphatidic acid receptor in gastric surface mucous cells: Possible implication of prostaglandin E2 production
Type 2 lysophosphatidic acid receptor in gastric surface mucous cells: Possible implication of prostaglandin E2 production
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胃表面粘液细胞中的 2 型溶血磷脂酸受体：前列腺素 E2 产生的可能意义
DOI：
10.1002/biof.1147
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发表时间：
2014
期刊：
影响因子：
6
通讯作者：
Kiyoshi Satouchi and Akira Tokumura
中科院分区：
文献类型：
--
作者：
Tamotsu Tanaka;Mayumi Ohmoto;Katsuya Morito;Hiroki Kondo;Mai Urikura;Kiyoshi Satouchi and Akira Tokumura
溶血磷脂酸（LPA）是一种脂质介质，通过其特异性受体诱导各种细胞反应。最近，我们发现口服LPA和磷脂酸（PA）可改善应激或阿司匹林引起的胃损伤。然而，这些影响的机制尚未阐明。在这项研究中，我们检测了LPA对表达2型LPA受体（LPA2）的胃细胞系MKN74细胞中前列腺素（PG） e2生成的影响。当LPA处理细胞时，COX‐2 mRNA水平上调，而COX‐1 mRNA水平上调。当细胞被百日咳毒素（PTX）预处理时，LPA效应被消除，表明受体(s)与Gi偶联参与其中。LPA预处理MKN74细胞可增强钙离子载体A23187触发的pge2生成。PTX再次消除了LPA效应。利用抗LPA2抗体进行荧光免疫组化，发现小鼠胃黏膜表面黏液细胞（坑细胞）在质膜顶端侧表达LPA2。这些结果表明，饮食中的LPA或其消化可能通过激活LPA2来增强pge2的产生，从而促进胃粘膜上皮的完整性。©2013 BioFactors, 40(3): 355-361, 2014
Lysophosphatidic acid (LPA) is a lipid mediator that induces various cell responses via its specific receptors. Recently, we found that orally administered LPA and phosphatidic acid (PA) ameliorate stress‐ or aspirin‐induced stomach injury. However, the mechanisms underlying these effects have not been elucidated yet. In this study, we examined effect of LPA on prostaglandin (PG) E2production in MKN74 cells, a gastric cell‐line expressing type 2 LPA receptor (LPA2). When the cells were treated with LPA, the level of mRNA of COX‐2 but not COX‐1 was upregulated. The LPA effect was abolished when the cells were pretreated with pertussis toxin (PTX), suggesting the involvement of receptor(s) coupled with Gi. Pretreatment of MKN74 cells with LPA enhanced the PGE2production triggered by calcium ionophore A23187. Again, PTX abolished the LPA effect. Fluorescent immunohistochemistry using an antibody against LPA2 showed that surface mucous cells (pit cells) in gastric mucosa of mice express LPA2 on the apical side of the plasma membrane. These results suggest that LPA in the diet or its digestion may contribute to the epithelial integrity of stomach mucosa by enhancement of PGE2production via activation of LPA2. © 2013 BioFactors, 40(3):355–361, 2014