Indication of circadian oscillations in the rat pancreas
Indication of circadian oscillations in the rat pancreas
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DOI：
10.1016/s0014-5793(04)00322-9
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发表时间：
2004-04-23
期刊：
影响因子：
3.5
通讯作者：
Peschke, E
中科院分区：
文献类型：
--
作者：
Mühlbauer, E;Wolgast, S;Peschke, E
视交叉上核的中央昼夜节律振荡器控制着身体的昼夜节律性，光是它的主要授时因子。最近，在肝脏和心脏中检测到了外围振荡器，这是尚未确定的线索。本研究采用实时荧光定量RT-PCR技术，检测了大鼠胰腺组织中主要生物钟基因Per 1、Per 2、Bmal 1、Cry 1、Tim（time-less）和Clock以及输出基因Dbp和Rev-erbalpha的表达。这里提出的结果表明一个强大的昼夜节律表达的时钟基因（例如Per 1和Bmal 1）和胰腺中可能存在的外周振荡器。这个振荡器是否调节胰岛的各种功能仍有待阐明。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The central circadian oscillator of the suprachiasmatic nucleus controls diurnal rhythmicity of the body with light as its dominant zeitgeber. Recently, peripheral oscillators have been detected in liver and heart, which follow as yet unidentified cues. In this study real-time reverse transcription-polymerase chain reaction (RT-PCR) was used in analysis of the expression of the major clock genes Per1, Per2, Bmal1, Cry1, Tim (time-less) and Clock, as well as of the output genes Dbp and Rev-erbalpha in the pancreatic tissue of rats. The results presented here indicate a robust circadian expression of clock genes (e.g. Per1 and Bmal1) and the probable existence of a peripheral oscillator in the pancreas. Whether this oscillator regulates the diverse functions of the islets of Langerhans remains to be elucidated. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.