Production of podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor
Production of podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor
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DOI：
10.1016/s1389-1723(02)80017-2
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发表时间：
2002-02-01
影响因子：
2.8
通讯作者：
Bisaria, VS
中科院分区：
文献类型：
--
作者：
Chattopadhyay, S;Srivastava, AK;Bisaria, VS
在装有低剪切 Setric 叶轮的 3 升搅拌罐生物反应器中进行了用于生产鬼臼毒素的鬼臼水下培养。为悬浮培养物中六枚藻的生长和鬼臼毒素的产生确定了培养基的具体要求，例如碳源（糖）和光。用葡萄糖替代蔗糖导致更高的生长和鬼臼毒素产量。当植物细胞在黑暗中培养时，有利于鬼臼毒素的生物合成。 100 rpm 的搅拌速度足以混合生物反应器中的培养液，而不会造成任何明显的细胞损伤。在分批生长条件下，3×1生物反应器中22天的生物量和鬼臼毒素累积量分别为6.5g/l和4.26mg/l。鬼臼毒素的总生产率为 0.19 mg/(l.d)，与摇瓶中的生产率相比增加了 27%。鬼臼毒素的产生被发现是一个与生长相关和与非生长相关的过程相结合的过程。
Submerged cultivation of Podophyllum hexandrum for the production of podophyllotoxin was carried out in a 3 l stirred tank bioreactor fitted with a low-shear Setric impeller. The specific requirements of the medium, such as carbon source (sugar) and light, were established for the growth of and podophyllotoxin production by P hexandrum in suspension cultures. Substitution of sucrose by glucose resulted in higher growth and podophyllotoxin production. The biosynthesis of podophyllotoxin was favored when plant cells were cultivated in the dark. An agitation speed of 100 rpm was sufficient to mix the culture broth in the bioreactor without causing any significant cell damage. Biomass and podophyllotoxin accumulation in 3 1 bioreactor under batch growth conditions were 6.5 g/l and 4.26 mg/l, respectively, in 22 d. This resulted in an overall podophyllotoxin productivity of 0.19 mg/(l.d), which represented an increase of 27% in comparison to its productivity in a shake flask. Podophyllotoxin production was found to be a combined growth-associated and non-growth associated process.