FPRP, a major, highly stoichiometric, highly specific CD81 and CD9-associated protein
FPRP, a major, highly stoichiometric, highly specific CD81 and CD9-associated protein
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DOI：
10.1074/jbc.m009859200
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发表时间：
2001-02-16
影响因子：
4.8
通讯作者：
Hemler, ME
中科院分区：
文献类型：
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作者：
Stipp, CS;Orlicky, D;Hemler, ME
CD 81和CD 9是跨膜-4超家族（TM 4SF;四跨膜蛋白）的成员，与其他TM 4SF蛋白、整联蛋白和其他蛋白形成广泛的复合物，特别是在温和的洗涤剂中。在中等严格的Brij 96裂解条件下，CD 81和CD 9复合物实际上彼此相同，但明显不同于其他TM 4SF复合物。CD 81和CD 9复合物中最突出的蛋白质之一在此被鉴定为FPRP，133-kDa前列腺素F-2 α受体调节蛋白。FPRP是一种细胞表面IG超家族蛋白，与CD 81或CD 81和CD 9特异性结合，但不与整联蛋白或其他TM 4 SF蛋白结合。与其他CD 81和CD 9相关蛋白相比，FPRP以非常高的化学计量结合，293细胞上基本上100%的细胞表面FPRP是CD 81和CD 9相关的。此外，CD81.CD9.FPRP复合物具有离散的大小（
CD81 and CD9, members of the transmembrane-4 superfamily (TM4SF; tetraspanins), form extensive complexes with other TM4SF proteins, integrins, and other proteins, especially in mild detergents. In moderately stringent Brij 96 lysis conditions, CD81 and CD9 complexes are virtually identical to each other, but clearly distinct from other TM4SF complexes. One of the most prominent proteins within CD81 and CD9 complexes is identified here as FPRP, the 133-kDa prostaglandin F-2 alpha receptor regulatory protein. FPRP, a cell-surface Ig superfamily protein, associates specifically with CD81 or with CD81 and CD9, but not with integrins or other TM4SF proteins. In contrast to other CD81- and CD9-associating proteins, FPRP associates at very high stoichiometry, with essentially 100% of cell-surface FPRP on 293 cells being CD81- and CD9-associated. Also, CD81.CD9.FPRP complexes have a discrete size (