Effect of Bacterial Infection on the Expression of Stress Proteins and Antioxidative Enzymes in Japanese Flounder
Effect of Bacterial Infection on the Expression of Stress Proteins and Antioxidative Enzymes in Japanese Flounder
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DOI：
10.1007/978-3-030-43484-7_8
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
T. Nakano;K. Osatomi;N. Miura;Yoko Aikawa-Fukuda;K. Kanai;Asami Yoshida;H. Shirakawa;Akiko Yamauchi;Toshiyasu Yamaguchi;Y. Ochiai
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文献类型：
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作者：
T. Nakano;K. Osatomi;N. Miura;Yoko Aikawa-Fukuda;K. Kanai;Asami Yoshida;H. Shirakawa;Akiko Yamauchi;Toshiyasu Yamaguchi;Y. Ochiai
人们认为，污染物和感染等环境刺激引起的压力会影响鱼类的健康。迟缓爱德华氏菌是商业鱼类的一种剧毒细胞内病原体。由E.引起的爱德华氏菌病。鲆鱼是牙鲆牙鲆最严重的疾病之一。然而，人们对细菌（如迟缓大肠杆菌）感染引起的应激对鱼类应激蛋白和抗氧化酶表达的影响知之甚少。在本研究中，我们检测了日本比目鱼肝胰腺中热休克蛋白 (HSP) 70 和超氧化物歧化酶 (SOD) 的表达，以响应大肠杆菌。迟发性感染。鱼类感染大肠杆菌 48 小时后，HSP70 表达迅速增加，显着升高。 tarda，与对照鱼相比。受感染鱼中 Cu、Zn-SOD 的表达水平从 24 小时增加到 48 小时，并在感染后 48 小时达到峰值。另一方面，感染鱼中 Mn-SOD 的表达水平从感染后 12 小时开始逐渐增加，并在感染后 24 至 48 小时内保持较高水平。 HSP70 和 SOD 表达水平的变化表明日本牙鲆存在活性氧诱导的氧化应激。结果还表明 HSP70 和 SOD 在介导大肠杆菌的免疫反应中发挥作用。日本比目鱼的迟缓感染。我们假设这些蛋白质是保护细胞免受氧化应激的重要因素。
Stress induced by environmental stimuli, such as pollutants and infection, in fish is thought to influence their fitness. BacteriumEdwardsiella tardais a virulent intracellular pathogen of commercial fish species. Edwardsiellosis caused byE. tardais one of the most severe diseases in Japanese flounderParalichthys olivaceus. However, little is known about the effect of bacterial (such asE. tarda) infectious-induced stress on the expression of stress proteins and antioxidative enzymes in fish. In the present study, we examined the expression of heat shock protein (HSP) 70 and superoxide dismutases (SODs) in the hepatopancreas of Japanese flounder in response toE. tardainfection. HSP70 expression was rapidly increased and was significantly higher in fish 48 h after being infected withE. tarda, compared to that in control fish. The expression level of Cu, Zn–SOD in infected fish increased from 24 to 48 h and peaked at 48 h post-infection. On the other hand, the expression level of Mn–SOD in infected fish gradually increased from 12 h post-infection and remained high between 24 and 48 h post-infections. The changes in expression levels of HSP70 and SODs are suggestive of reactive oxygen species-induced oxidative stress in Japanese flounder. The results also suggest that both HSP70 and SODs play roles in mediating immune response uponE. tardainfection in Japanese flounder. We hypothesize that these proteins are important factors that protect cells against oxidative stress.