FbpC is not essential for iron acquisition in Neisseria gonorrhoeae.
FbpC is not essential for iron acquisition in Neisseria gonorrhoeae.
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FbpC 对于淋病奈瑟菌的铁获取不是必需的。
DOI：
10.1128/iai.67.6.3141-3145.1999
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发表时间：
1999
影响因子：
3.1
通讯作者：
Genco,CA
中科院分区：
文献类型：
--
作者：
Sebastian,S;Genco,CA
淋病奈瑟菌的 fbpABC 基因座已被提议编码周质蛋白依赖性铁转运系统。尽管淋球菌 FbpA 蛋白的功能已得到充分表征，并且其作为周质结合蛋白的作用也已明确，但对 FbpB 和 FbpC 蛋白的功能知之甚少。定义淋球菌 FbpC 蛋白 anN 的功能。淋病F62fbpC突变体是通过卡那霉素基因插入失活构建的。然后。观察到淋病菌 F62fbpC 突变体在血红素、转铁蛋白或硝酸铁作为唯一外源铁源的情况下生长，表明淋球菌 FbpC 蛋白对于这些铁源的生长并不是绝对必需的。在之前的研究中，我们无法通过 Northern 分析检测 fbpB- 或 fbpC 特异性转录本。使用从 N 获得的 RNA 进行逆转录 PCR 分析。在铁充足和贫铁条件下生长的淋病菌F62检测到fbpA和fbpAB转录本，但未能检测到fbpCorfbpBC转录本。这些结果表明 FbpC 在 N 中的铁转运中并不发挥关键作用。淋球菌并表明额外的 ABC 转运系统在淋球菌中具有获取铁的功能。
ThefbpABClocus ofNeisseria gonorrhoeaehas been proposed to encode a periplasmic protein-dependent iron transport system. Although the function of the gonococcal FbpA protein has been well characterized and its role as a periplasmic binding protein is well defined, little is known about the function of the FbpB and FbpC proteins. To define the function of the gonococcal FbpC protein, anN. gonorrhoeaeF62fbpCmutant was constructed by insertional inactivation with the kanamycin gene. TheN. gonorrhoeaeF62fbpCmutant was observed to grow with heme, transferrin, or ferric nitrate as the sole exogenous iron source, indicating that the gonococcal FbpC protein is not absolutely required for growth with these iron sources. In previous studies we were unable to detectfbpB- orfbpC-specific transcripts by Northern analysis. Reverse transcription-PCR analysis with RNA obtained fromN. gonorrhoeaeF62 grown under iron-replete and -depleted conditions detectedfbpAandfbpABtranscripts but failed to detectfbpCorfbpBCtranscripts. These results indicate that FbpC does not play a pivotal role in iron transport inN. gonorrhoeaeand suggest that additional ABC transport systems are functional in the gonococcus for the acquisition of iron.