Microplastic ingestion perturbs the microbiome of Aedes albopictus (Diptera: Culicidae) and Aedes aegypti
Microplastic ingestion perturbs the microbiome of Aedes albopictus (Diptera: Culicidae) and Aedes aegypti
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DOI：
10.1093/jme/tjad097
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发表时间：
2023-07-21
影响因子：
2.1
通讯作者：
Brelsfoard，Corey L.
中科院分区：
文献类型：
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作者：
Edwards，Carla-Cristina;McConnel，Gabriella;Brelsfoard，Corey L.
微塑料(MPS)是一种常见的环境污染物，然而，人们对其被昆虫摄入后的影响知之甚少。在这里，我们饲养了埃及伊蚊(Stegmyia)。和白纹伊蚊(Skuse)蚊子幼虫1微米聚苯乙烯MPS，并检测摄食对成虫羽化率、肠道损伤以及真菌和细菌微生物群的影响。结果表明，MPS在幼虫肠道中积累，导致肠道损伤。然而，对成虫羽化率的影响很小。在羽化阶段羽化后的成虫肠道中也发现了MPS，成虫在获得糖餐后会在其兄弟会中排出MPS。此外，MPS对昆虫微生物群的影响需要更好地定义。为了解决这一知识差距，我们调查了MP摄入量与INAE微生物群落之间的关系。AlbopictusandAe.埃及伊蚊。随着MPS浓度的增加，微生物区系组成发生了变化。导致MP处理之间细菌和真菌微生物区系组成差异的扩增子序列变体(ASV)分别来自伊丽莎白属和曲霉属。此外，在幼虫摄取MPS的处理中，观察到真菌和细菌微生物区系的α多样性降低。这些结果突显了蚊子微生物群中的细菌和真菌成分对MPS的摄入做出不同反应的可能性。根据我们的发现以及MP摄入对蚊子宿主微生物和真菌生物群的影响，MP污染可能会影响重要的蚊媒病毒和寄生虫的媒介能力，这些病毒和寄生虫会导致人和动物疾病。
Microplastics (MPs) are common environmental pollutants; however, little is known about their effects after ingestion by insects. Here we fedAedes(Stegomyia)aegypti(L.) andAedes(Stegomyia)albopictus(Skuse) mosquito larvae 1 µm polystyrene MPs and examined the impacts of ingestion on adult emergence rates, gut damage, and fungal and bacterial microbiota. Results show that MPs accumulate in the larval guts, resulting in gut damage. However, little impact on adult emergence rates was observed. MPs are also found in adult guts postemergence from the pupal stage, and adults expel MPs in their frass after obtaining sugar meals. Moreover, MPs effects on insect microbiomes need to be better defined. To address this knowledge gap, we investigated the relationship between MP ingestion and the microbial communities inAe. albopictusandAe. aegypti. The microbiota composition was altered by the ingestion of increasing concentrations of MPs. Amplicon sequence variants (ASVs) that contributed to differences in the bacterial and fungal microbiota composition between MP treatments were from the generaElizabethkingiaandAspergillus, respectively. Furthermore, a decrease in the alpha diversity of the fungal and bacterial microbiota was observed in treatments where larvae ingested MPs. These results highlight the potential for the bacterial and fungal constituents in the mosquito microbiome to respond differently to the ingestion of MPs. Based on our findings and the effects of MP ingestion on the mosquito host micro- and mycobiome, MP pollution could impact the vector competence of important mosquito-transmitted viruses and parasites that cause human and animal diseases.