Development of Surface Molecularly Imprinted Polymers as Dispersive Solid Phase Extraction Coupled with HPLC Method for the Removal and Detection of Griseofulvin in Surface Water
Development of Surface Molecularly Imprinted Polymers as Dispersive Solid Phase Extraction Coupled with HPLC Method for the Removal and Detection of Griseofulvin in Surface Water
复制标题
表面分子印迹聚合物分散固相萃取联合高效液相色谱法去除和检测地表水中灰黄霉素的研究
DOI：
10.3390/ijerph17010134
复制
发表时间：
2020-01-01
影响因子：
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通讯作者：
Fu, Qiang
中科院分区：
文献类型：
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作者：
Bashir, Kamran;Luo, Zhimin;Fu, Qiang
灰黄霉素（GSF）在临床上用于治疗人类和动物的真菌感染。在地表沃茨中检测到GSF作为药物污染物。GSF作为一种人为污染物被认为是生态系统中耐药性和风险因素的可能来源。为了解决这个问题，开发了一种新的提取和富集方法。制备了GSF表面分子印迹聚合物（GSF-SMIPs），并将其应用于固相萃取（SPE）。建立了分散固相萃取（DSPE）-高效液相色谱法测定地表水中GSF的方法。评估了GSF-SMIP的性能，以确定其从水样中去除GSF的潜力。考察了样品pH值、离子强度等因素对去除效果的影响，并进行了优化。对GSF-SMIPs用量、萃取时间、解吸溶剂种类和体积等DSPE条件进行了优化。所建立的方法在0.1-100 µg/mL范围内呈线性。检测限和定量限分别为0.01和0.03 µg/mL。DSPE后回收率良好（91.6-98.8%）。日内和日间相对标准偏差分别为0.8%和4.3%。与粉末活性炭（67.7%）相比，SMIP表现出良好的去除效率（91.6%）。此外，SMIP可以重复使用10次水样。这是一个额外的优势，超过一次性使用的活性炭和其他商业吸附剂。本研究为地表水样品中GSF的选择性提取和检测提供了一种特异、灵敏的方法。
Griseofulvin (GSF) is clinically employed to treat fungal infections in humans and animals. GSF was detected in surface waters as a pharmaceutical pollutant. GSF detection as an anthropogenic pollutant is considered as a possible source of drug resistance and risk factor in ecosystem. To address this concern, a new extraction and enrichment method was developed. GSF-surface molecularly imprinted polymers (GSF-SMIPs) were prepared and applied as solid phase extraction (SPE) sorbent. A dispersive solid phase extraction (DSPE) method was designed and combined with HPLC for the analysis of GSF in surface water samples. The performance of GSF-SMIPs was assessed for its potential to remove GSF from water samples. The factors affecting the removal efficiency such as sample pH and ionic strength were investigated and optimized. The DSPE conditions such as the amount of GSF-SMIPs, the extraction time, the type and volume of desorption solvents were also optimized. The established method is linear over the range of 0.1–100 µg/mL. The limits of detection and quantification were 0.01 and 0.03 µg/mL respectively. Good recoveries (91.6–98.8%) were achieved after DSPE. The intra-day and inter-day relative standard deviations were 0.8 and 4.3% respectively. The SMIPs demonstrated good removal efficiency (91.6%) as compared to powder activated carbon (67.7%). Moreover, the SMIPs can be reused 10 times for water samples. This is an additional advantage over single-use activated carbon and other commercial sorbents. This study provides a specific and sensitive method for the selective extraction and detection of GSF in surface water samples.