Insights Into the Role of Extracellular DNA and Extracellular Proteins in Biofilm Formation of Vibrio parahaemolyticus
Insights Into the Role of Extracellular DNA and Extracellular Proteins in Biofilm Formation of Vibrio parahaemolyticus
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DOI：
10.3389/fmicb.2020.00813
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发表时间：
2020-05-19
影响因子：
5.2
通讯作者：
Zhao, Yong
中科院分区：
文献类型：
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作者：
Li, Wei;Wang, Jing Jing;Zhao, Yong
细胞外聚合物(EPS)构成了生物膜的三维结构，但它们各自的作用尚不清楚。因此，本研究旨在阐明EPS的关键化学成分[胞外DNA、胞外蛋白和碳水化合物]在副溶血性弧菌生物膜形成中的作用。首次确定了各关键化学成分与生物膜形成的相关性，发现副溶血弧菌的生物膜形成与EDNA和蛋白质含量均呈极显著正相关(P<；0.01)，而与碳水化合物含量无相关性。随后，单独的DNase I或蛋白酶K处理显著降低了形成的生物膜的初始粘附性和结构稳定性，但没有引起成熟生物膜的显著分散。然而，DNase I和蛋白酶K的联合处理通过同时破坏EDNA和胞外蛋白，导致成熟生物膜明显分散。在结构水平上的分析表明，生物膜的崩溃主要是由于酶处理对EDNA的环状构型和蛋白质的二级结构造成了很大的破坏。因此，本研究对EPS的关键化学成分在副溶血性弧菌生物膜形成中的作用有了深入的了解，这可能为开发环境友好的方法根除食品工业中的生物膜提供新的策略。
The extracellular polymeric substances (EPS) construct the three-dimensional (3-D) structure of biofilms, but their respective roles are still not clear. Therefore, this study aimed to illuminate the role of key chemical components [extracellular DNA (eDNA), extracellular proteins, and carbohydrates] of EPS in biofilm formation of Vibrio parahaemolyticus. The correlations between each key chemical component and biofilm formation were first determined, showing that the biofilm formation of V. parahaemolyticus was strongly positively correlated with both eDNA and protein content (P < 0.01), but not with carbohydrates. Subsequently, individual DNase I or protease K treatment markedly reduced the initial adhesion and structural stability of the formed biofilms by hydrolyzing the eDNA or extracellular proteins, but did not induce significant dispersion of mature biofilms. However, the combination of DNase I and protease K treatment induced the obvious dispersion of the mature biofilms through the concurrent destruction of eDNA and extracellular proteins. The analysis at a structural level showed that the collapse of biofilms was mainly attributed to the great damage of the loop configuration of eDNA and the secondary structure of proteins caused by the enzyme treatment. Therefore, this study provides a deep understanding of the role of key chemical components of EPS in biofilm development of V. parahaemolyticus, which may give a new strategy to develop environmentally friendly methods to eradicate the biofilms in food industry.