Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity
Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity
复制标题
分析精子来源的环境 DNA 的持久性和粒径分布变化以监测竹荚鱼产卵活动
DOI：
10.1021/acs.est.2c01904
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Masuda Reiji
中科院分区：
文献类型：
--
作者：
Tsuji Satsuki;Murakami Hiroaki;Masuda Reiji
环境DNA（eDNA）分析作为一种有效和非侵入性的方法，不仅可以监测生物的分布，而且可以监测它们的产卵活动，具有很大的前景。在基于eDNA分析的产卵活动监测中，精子来源的eDNA的检测是一个关键点;然而，其特征和动力学是完全未知的。本文研究了日本竹荚鱼精子cDNA的持久性和粒径分布。首先，我们研究了人工添加精子到海水中的精子来源的eDNA的时间依赖性降解和PSD。接下来，我们把鱼放在水箱里，检查产卵前后eDNA浓度和PSD的变化。两个实验的结果表明，精子来源的eDNA的降解进行迅速，PSD转移到一个较小的大小，无论DNA区域（Cytbor ITS 1）。此外，研究表明，通过降解从精子中释放的细胞核和线粒体的大小分布不仅仅取决于每个细胞器的大小。这些结果将有助于阐明特别是在产卵季节的eDNA的特性和动态，并进一步发展eDNA分析作为一个强大的工具，用于监测产卵活动。
Environmental DNA (eDNA) analysis holds great promise as an efficient and noninvasive method to monitor not only the distribution of organisms but also their spawning activity. In eDNA analysis-based monitoring of spawning activity, the detection of sperm-derived eDNA is a key point; however, its characteristics and dynamics are completely unknown. The present study focuses on the persistence and particle size distribution (PSD) of eDNA derived from the sperm of Japanese jack mackerel. First, we investigated the time-dependent degradation and the PSD of sperm-derived eDNA by artificially adding sperm to seawater. Next, we kept fish in tanks and examined the changes in eDNA concentration and PSD before and after spawning. The results of two experiments showed that the degradation of sperm-derived eDNA proceeded rapidly, with PSD shifting to a smaller size regardless of the DNA region (Cytbor ITS1). Additionally, it was shown that the nuclei and mitochondria released from sperm through degradation had a size distribution that was not simply dependent on each organelle size. These results will contribute to elucidating the characteristics and dynamics of eDNA specifically during the spawning season and to further developing eDNA analysis as a powerful tool for the monitoring of spawning activity.