VASCULAR LEAKAGE IN DORSAL ROOT-GANGLIA OF RAT, STUDIES WITH HORSERADISH-PEROXIDASE
VASCULAR LEAKAGE IN DORSAL ROOT-GANGLIA OF RAT, STUDIES WITH HORSERADISH-PEROXIDASE
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DOI：
10.1016/0022-510x(76)90083-6
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发表时间：
1976-01-01
影响因子：
4.4
通讯作者：
CAVANAGH, JB
中科院分区：
文献类型：
--
作者：
JACOBS, JM;MACFARLANE, RM;CAVANAGH, JB
在大鼠静脉注射后2 min内，辣根过氧化物酶（HRP）[随后是EM]从神经节血管进入背根和Vth神经节的细胞外间隙。到5分钟，示踪剂已渗透到神经元周围和轴突周围空间。发生了活跃的巨噬细胞反应，这些细胞迅速吞噬示踪剂，因此在2-3小时内示踪剂几乎完全从细胞外空间消失。在神经节内可见有孔血管，有或无横膈膜。根据HRP固定后灌注的示踪剂分布模式，开窗和内皮细胞间裂隙可能是示踪剂渗漏的最重要途径。由于示踪剂的外源性和可能的毒性，其运动和命运可能与正常外渗的蛋白质不平行。
Horseradish peroxidase (HRP) [as followed by EM] passed from ganglionic blood vessels into the extracellular space of dorsal root and Vth nerve ganglia within 2 min of i.v. injection in the rat. By 5 min the tracer had penetrated into perineuronal and periaxonal spaces. A brisk macrophage response occurred, and these cells rapidly engulfed the tracer, so that it had almost completely disappeared from the extracellular space by 2-3 h. Fenestrated blood vessels, with and without diaphragms, were seen within ganglia. On the basis of the pattern of tracer distribution following post-fixation perfusion of HRP, the fenestrations and endothelial intercellular clefts were probably the most important route of tracer leakage. Because of the foreign and possibly toxic nature of the tracer, its movements and fate may not parallel that of normally extravasated proteins.