Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans isolated from a population of periodontitis patients in Spain
Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans isolated from a population of periodontitis patients in Spain
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DOI：
10.1016/j.archoralbio.2014.07.021
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发表时间：
2014-12-01
影响因子：
3
通讯作者：
Sanz, Mariano
中科院分区：
文献类型：
--
作者：
Minguez, Maria;Pousa, Xiana;Sanz, Mariano
目的：尚无研究描述西班牙牙周炎患者中放线菌伴生聚集菌分离株的变异性。因此，本次调查的目的是研究从西班牙牙周炎患者中分离出的 A. actinomycetemcomitans 菌株的血清型分布。还将研究编码白细胞毒素和细胞致死膨胀毒素 (cdt) 操纵子的基因的多态性。 设计：在总共 701 名患者样本中，40 名放线放线菌阳性牙周炎患者被纳入研究（平均年龄 45.3 岁，62.5% 为女性），并对他们的临床牙周状况进行了评估。平均而言，对每位患者的 1-3 个分离株进行传代培养并通过 PCR 进行表征。结果：使用培养物，A. actinomycetemcomitans 的患病率为 5.7%。最常见的血清型是“b”，即 30 名患者被一种独特的血清型感染，而 7 名患者表现出共同定植，其中大多数为“a”和“b”血清型。从获得的79个纯分离株中，24个来自血清型“a”，30个来自血清型“b”，12个来自血清型“c”，4个来自血清型“d”。这些样品的进一步表征表明，这 79 个分离株中没有一个显示出 JP2 菌株特征的白细胞毒素启动子区域中的 530 bp 缺失。相反，65.8%的分离株为cdt+。结论：最常见的血清型是“a”和“b”，其中血清型“b”在单定植中最常见，而血清型“e”和“f”未检测到。在大多数样本中，发现了编码 cdt 的操纵子（65.8%）和负责编码白细胞毒素的基因（100%）。没有一个分离株是 JP2 菌株。 (C) 2014 Elsevier Ltd. 保留所有权利。
Objective: There is no study characterizing the variability of Aggregatibacter actinomycetemcomitans isolates in periodontitis patients in Spain. It is therefore the aim of this investigation to study the serotype distribution of A. actinomycetemcomitans strains isolated from periodontitis patients in Spain. The polymorphism of the genes that codifies the leukotoxin and the operon of the cytolethal-distending toxin (cdt) will also be investigated.Design: From a total of 701 patients samples, 40 A. actinomycetemcomitans-positive periodontitis patients were included in the study (mean age 45.3, 62.5% females) and their clinical periodontal status was assessed. On average, 1-3 isolates from each patient were sub-cultured and characterized by PCR.Results: Using culture the prevalence of A. actinomycetemcomitans was 5.7%. The most frequent serotype was "b", being 30 patients infected by a unique serotype, while 7 patients showed co-colonization, mostly with serotypes "a" and "b". From the 79 pure isolates obtained, 24 were from serotype "a", 30 from serotype "b", 12 from serotype "c" and 4 from serotype "d". Further characterization of these samples showed that none of these 79 isolates demonstrated the 530-bp deletion in the leukotoxin's promoter region that characterizes the JP2 strain. Conversely 65.8% of the isolates were cdt+.Conclusions: The most common serotypes were "a" and "b", being serotype "b" the most prevalent in mono-colonization, while serotypes "e" and "f" were not detected. In the majority of samples, operon that codifies the cdt (65.8%) and the genes responsible for the codification of leukotoxin (100%) were found. None of the isolates were JP2 strains. (C) 2014 Elsevier Ltd. All rights reserved.