Reply to "Understanding the Role of Fungi in Chronic Wounds".
Reply to "Understanding the Role of Fungi in Chronic Wounds".
复制标题
回复“了解真菌在慢性伤口中的作用”。
DOI：
10.1128/mbio.02033-16
复制
发表时间：
2016
期刊：
影响因子：
6.4
通讯作者：
Grice,ElizabethA
中科院分区：
文献类型：
--
作者：
Kalan,Lindsay;Gardner,SueE;Grice,ElizabethA
用来采集样本进行微生物组分析的技术非常重要。所有的下游处理和分析都依赖于准确可靠的样品。马龙和迪克森是正确的，因为其他小组主张使用更具侵入性的方法，尽管文献中缺乏严格的、同行评议的证据来表明这些侵入性技术的优越性。有趣的是，我们注意到马龙撰写了一篇论文，《深度伤口培养与糖尿病足骨髓炎的骨活检培养有很好的相关性》(3)，其中的结果似乎与信中提出的论点相矛盾。尽管如此，我们非常感谢有机会进一步阐述我们使用Levine技术收集拭子样本的原因。Levine的技术与其他拭子技术的不同之处在于，它从深层组织层采样液体，类似于伤口液体的抽吸。在这种方法中，伤口用非抑菌盐水清洗，拭子在1平方厘米的活的、非坏死性的伤口组织上旋转，以足够的压力机械地破坏与生物膜和样本生物相关的多糖基质，以供S使用。我们使用Levine的技术而不是获取组织来获取标本的原因如下。
The technique used to collect a sample for microbiome analysis is of great importance. All downstream processing and analysis relies on an accurate and reliable sample. Malone and Dickson are correct in that other groups advocate using more-invasive methods, though rigorous, peer-reviewed evidence to suggest the superiority of those invasive techniques is lacking in the literature. Interestingly, we noted that Malone authored a paper,“Deep Wound Cultures Correlate Well with Bone Biopsy Culture in Diabetic Foot Osteomyelitis”(3), in which the results seemingly contradict the argument presented in the letter. Nonetheless, we are most appreciative for the opportunity to further expand on our reasons for employing the Levine technique for collecting swab specimens.Levine’s technique is different than other swab techniques in that it samples fluid from deep tissue layers, similarly to aspiration of wound fluid. In this method, the wound is cleansed with nonbacteriostatic saline and a swab is rotated over a 1-cm2 area of viable, nonnecrotic wound tissue for 5 s with sufficient pressure to mechanically disrupt the polysaccharide matrix associated with biofilms and sample organisms. Our reasons for employing Levine’s technique, as opposed to obtaining tissue, for specimen acquisition, are as follows.