Universal primers for species authentication of animal foodstuff in a single polymerase chain reaction
Universal primers for species authentication of animal foodstuff in a single polymerase chain reaction
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DOI：
10.1002/jsfa.5766
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发表时间：
2013-01-30
影响因子：
4.1
通讯作者：
Garcia-Vazquez, Eva
中科院分区：
文献类型：
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作者：
Horreo, Jose L.;Ardura, Alba;Garcia-Vazquez, Eva
背景：有许多基于 DNA 的系统用于检测食品和食品中存在的动物物种，适用于食品质量控制和认证。然而，大多数（如果不是全部）方法需要一对以上的引物，并且不能应用于广泛的分类范围，例如。 g。使用相同的引物和方案识别脊椎动物和无脊椎动物。结果：此处描述的一对引物允许在单个聚合酶链式反应中识别食品和加工（预煮、罐装或熏制）食品中广泛分类范围的动物物种。结论：这些引物可以识别人类营养中使用的大多数动物分类群，从软体动物等无脊椎动物到高等脊椎动物，区分同一属的物种。 16S rDNA 内扩增的短片段具有系统发育价值，并且根据所分析的广泛分类范围可以被认为具有普遍性。这些引物易于使用，对于预算有限的实验室来说也很容易获得，而且对于消费者信息和揭露食品欺诈也很有价值。 (C)2012年化学工业学会
BACKGROUND: There are many DNA-based systems for detecting animal species present in food and food products, applicable for food quality control and authentication. However, most ( if not all) methods require more than one pair of primers and cannot be applied over a wide taxonomic range, e. g. identifying vertebrates and invertebrates with the same primers and protocols.RESULTS: A pair of primers is described here that allows in a single polymerase chain reaction the identification of animal species in food and processed ( precooked, canned or smoked) food products over a wide taxonomic range.CONCLUSION: These primers permit the identification of most animal taxa employed in human nutrition, from invertebrates such as molluscs to higher vertebrates, distinguishing between species of the same genus. The short fragment amplified within the 16S rDNA exhibits phylogenetic value and could be considered universal based on the wide taxonomic range assayed. The primers are easy to use and accessible for laboratories with a modest budget, as well as being valuable for consumer information and to reveal food fraud. (C) 2012 Society of Chemical Industry