Antibacterial effects of three experimental quaternary ammonium salt (QAS) monomers on bacteria associated with oral infections.
Antibacterial effects of three experimental quaternary ammonium salt (QAS) monomers on bacteria associated with oral infections.
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DOI：
10.2334/josnusd.50.323
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发表时间：
2008-09-01
影响因子：
1.9
通讯作者：
Li, Fang
中科院分区：
文献类型：
--
作者：
Xiao, Yu-Hong;Chen, Ji-Hua;Li, Fang
本研究的目的是测试三种实验性季铵盐单体的抗菌效果，以评估其作为牙科材料的潜在应用。通过肉汤稀释法对口腔感染相关细菌进行单体的体外敏感性试验：变形链球菌ATCC 25175、粘性放线菌ATCC 15987、金黄色葡萄球菌ATCC 29213和干酪乳杆菌ATCC 393。研究了抗菌活性较高的单体对S.还研究了变异体。结果发现，所有测试的细菌菌株都对三种单体敏感，其中甲基丙烯酰氧基乙基十六烷基氯化铵（DMAE-CB）表现出最低的最小抑菌浓度，范围为1.2至4.8微克/毫升。时间-杀灭曲线显示，DMAE-CB在19.2 μ g/ml（最低杀菌浓度的4倍）时对沙门氏菌的杀灭率为99.44%。在接触1分钟后100%杀死变形杆菌，在接触10分钟内100%杀死变形杆菌。这一结果表明，季铵盐单体DMAE-CB可能是一个候选的抗菌剂纳入牙科修复材料。
The aim of this study was to test the antibacterial effects of three experimental quaternary ammonium salt monomers in order to evaluate their potential applications as dental materials. In vitro susceptibility testing of the monomers was performed by the broth dilution method on bacteria associated with oral infections: Streptococcus mutans ATCC 25175, Actinomyces viscosus ATCC 15987, Staphylococcus aureus ATCC 29213 and Lactobacillus casei ATCC 393. The time-kill kinetics of the monomer with relatively higher antibacterial activity against S. mutans were also investigated. It was found that all the tested bacteria strains were susceptible to the three monomers, among which methacryloxylethyl cetyl ammonium chloride (DMAE-CB) exhibited the lowest minimal inhibitory concentrations, ranging from 1.2 to 4.8 microg/ml. The time-kill curve showed that DMAE-CB achieved 99.44% killing at 19.2 microg/ml (4 times the minimal bactericidal concentration) against S. mutans after 1 min and 100% killing within 10 min of contact. This result indicates that the quaternary ammonium salt monomer DMAE-CB may be a candidate antibacterial agent for incorporation into dental restorative materials.