Comparison of Endothelial Cell Phenotypic Markers of Late-Outgrowth Endothelial Progenitor Cells Isolated from Patients with Coronary Artery Disease and Healthy Volunteers
Comparison of Endothelial Cell Phenotypic Markers of Late-Outgrowth Endothelial Progenitor Cells Isolated from Patients with Coronary Artery Disease and Healthy Volunteers
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DOI：
10.1089/ten.tea.2008.0673
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发表时间：
2009-11-01
影响因子：
4.1
通讯作者：
Reichert, William M.
中科院分区：
文献类型：
--
作者：
Stroncek, John D.;Grant, Bryan S.;Reichert, William M.
缺乏容易分离的自体内皮细胞（EC）来源是血管组织工程干预的主要挑战之一。本文探讨了从50 mL的外周血样本中分离和扩增晚期生长内皮祖细胞（EPCs），这些样本来自患有显著冠状动脉疾病（CAD）的患者和健康的年轻成年志愿者。在成功分离晚期生长的EPCs的情况下，在体外测定细胞的EC标记物表达、增殖潜力和使合成材料内皮化、形成新血管和产生一氧化氮的能力。来自CAD患者和健康志愿者的晚期生长EPCs表现出关键的EC标记物和形态学特征，与人主动脉EC的对照人群相似。据我们所知，这是第一项研究，以检查适用于自体内皮化应用的CAD患者的晚期生长的EPCs。
The lack of easily isolated autologous endothelial cell (EC) sources is one of the major challenges with vascular tissue engineering interventions. This article examines the isolation and expansion of late-outgrowth endothelial progenitor cells (EPCs) from 50-mL samples of peripheral blood drawn from patients with significant coronary artery disease (CAD) and healthy young adult volunteers. In cases in which late-outgrowth EPCs were successfully isolated, the cells were assayed in vitro for their expression of EC markers, proliferation potential and ability to endothelialize synthetic materials, form new blood vessels, and produce nitric oxide. Late-outgrowth EPCs from patients with CAD and healthy volunteers exhibited critical EC markers and morphological characteristics that were analogous to a control population of human aortic ECs. To our knowledge, this is the first study to examine the suitability of late-outgrowth EPCs from patients with CAD for autologous endothelialization applications.