Microchip device for measurement of body volume of C. elegans as bioindicator application
Microchip device for measurement of body volume of C. elegans as bioindicator application
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DOI：
10.1007/s12213-011-0036-7
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发表时间：
2012-12
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影响因子：
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通讯作者：
Jaehoon Jung;M. Nakajima;M. Kojima;K. Ooe;T. Fukuda
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作者：
Jaehoon Jung;M. Nakajima;M. Kojima;K. Ooe;T. Fukuda
我们提出了一种微流控装置来分析秀丽隐杆线虫（C. elegans）作为生物指示剂。在该装置中，我们评估了C的身体体积。elegansusing使用a microchip芯片with a capacitive电容sensor传感器.该装置由两个关键部分组成。一种是锥形微通道。锥形微通道用于固定C。优雅的另一个是一对电极。电极用于测量电容变化。这两个元件形成一个感应空间;当C.放置在感应空间中的优雅。在这项研究中，我们进行了两种实验。首先，检查身体体积和电容变化之间的相关性，共15 C。从L_2幼虫期到成虫期，第二，确认所提出的微芯片作为分析C. elegansas为生物指示剂，共35 C.优雅是衡量。以测量身体体积为参考，首先测量C的体长和直径。通过光学显微镜测量，然后由这两个测量值计算体积。电容变化的测量所提出的微芯片。通过对两种方法计算结果的比较，证实了计算值之间的相关性。探讨了微芯片作为分析镉对C. elegansas生物指示剂的建立。
We propose a micro fluidic device to analyzeCaenorhabditis elegans(C. elegans) as a bioindicator. In this device, we assessed the body volume ofC. elegansusing a microchip with a capacitance sensor. This device is composed of two key parts. One is a tapered micro channel. The tapered micro channel is used to immobilizeC. elegans. The other is a pair of electrodes. The electrodes are used to measure the capacitance change. These two components form a sensing space; a capacitance change occurs whenC. elegansis placed in the sensing space. In this study, we performed two kinds of experiments. First, to check the correlation between the body volume and the capacitance change, a total of 15C. eleganswere measured; from L2 larval stage to adult stage. Second, to confirm the possibility of the proposed microchip as a device that can analyze the condition ofC. elegansas a bioindicator, a total of 35C. eleganswere measured. To measure the body volume as a reference, first, the body length and the diameter ofC. eleganswere measured by an optical microscope and then the volume was calculated from those two measured values. The capacitance change was measured by the proposed microchip. By comparison between the result of two methods, we confirmed the correlation between the calculated values. The possibility of the proposed microchip as the device to analyze the effect of cadmium onC. elegansas a bioindicator was established.