Capacitive sensing of triglyceride film reactions: a proof-of-concept demonstration for sensing in simulated duodenal contents with gastrointestinal targeting capsule system
Capacitive sensing of triglyceride film reactions: a proof-of-concept demonstration for sensing in simulated duodenal contents with gastrointestinal targeting capsule system
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DOI：
10.1039/d0lc00133c
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发表时间：
2020-06-07
期刊：
影响因子：
6.1
通讯作者：
Ghodssi，Reza
中科院分区：
文献类型：
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作者：
Banis，George E.;Beardslee，Luke A.;Ghodssi，Reza
可摄取胶囊系统继续发展以克服与传统胃肠道（GI）诊断和治疗过程相关联的缺点，例如对可以进入GI道的哪些部分的限制或不能测量局部生物标志物浓度。我们报告了一个集成的胶囊传感系统，利用混合包装方案加上甘油三酯薄膜涂层电容传感器，用于测量存在于十二指肠的生化物质，如胰脂肪酶和胆汁酸。该系统使用与蓝牙低功耗（BLE）微控制器接口的微制造电容传感器，允许无线连接到移动的应用程序。甘油三酯薄膜使传感器表面绝缘，并通过水解与0.01-1 mM脂肪酶反应，或通过在模拟流体中乳化与0.07- 7%w/v胆汁酸反应，导致电容的可测量变化。在磷酸盐缓冲盐水（PBS）以及胰蛋白酶溶液中评价甘油三酯膜的交叉反应性。在暴露于每个刺激之后观察膜形态，以更好地理解这些变化如何改变传感器电容。该胶囊利用3D打印包装，该包装涂覆有在酸性溶液中保持完整的聚合物（模拟胃条件），然后在模拟中性pH值的pH值下溶解，以触发传感室的打开，我们随后可以检测到胰脂肪酶的存在。该设备策略代表了使用嵌入式包装和基于聚碳酸酯的材料来靶向胃肠道的特定区域并感测生化含量以评估胃肠道健康的重要一步。
Ingestible capsule systems continue to evolve to overcome drawbacks associated with traditional gastrointestinal (GI) diagnostic and therapeutic processes, such as limitations on which sections of the GI tract can be accessed or the inability to measure local biomarker concentrations. We report an integrated capsule sensing system, utilizing a hybrid packaging scheme coupled with triglyceride film-coated capacitive sensors, for measuring biochemical species present in the duodenum, such as pancreatic lipase and bile acids. The system uses microfabricated capacitive sensors interfaced with a Bluetooth low-energy (BLE)-microcontroller, allowing wireless connectivity to a mobile app. The triglyceride films insulate the sensor surface and react either with 0.01–1 mM lipase via hydrolysis or 0.07–7% w/v bile acids via emulsification in simulated fluids, leading to measurable changes in capacitance. Cross reactivity of the triglyceride films is evaluated in both phosphate buffered saline (PBS) as well as pancreatic trypsin solutions. The film morphology is observed after exposure to each stimulus to better understand how these changes alter the sensor capacitance. The capsule utilizes a 3D-printed package coated with polymers that remain intact in acid solution (mimicking gastric conditions), then dissolve at a duodenum-mimicking neutral pH for triggered opening of the sensing chamber from which we can subsequently detect the presence of pancreatic lipase. This device strategy represents a significant step towards using embedded packaging and triglyceride-based materials to target specific regions of the GI tract and sensing biochemical contents for evaluating gastrointestinal health.