Amphiphilic phospholipid-iodinated polymer conjugates for bioimaging.
Amphiphilic phospholipid-iodinated polymer conjugates for bioimaging.
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DOI：
10.1039/d0bm02098b
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发表时间：
2021-06
影响因子：
6.6
通讯作者：
Richmond Djorgbenoo;Mac Michael M Rubio;Ziyu Yin;Keyori J Moore;A. Jayapalan;Joshua Fiadorwu;Boyce E. Collins;Brian Velasco;Kokougan Allado;J. Tsuruta;C. Gorman;Jianjun Wei;Kennita A. Johnson;P. He
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作者：
Richmond Djorgbenoo;Mac Michael M Rubio;Ziyu Yin;Keyori J Moore;A. Jayapalan;Joshua Fiadorwu;Boyce E. Collins;Brian Velasco;Kokougan Allado;J. Tsuruta;C. Gorman;Jianjun Wei;Kennita A. Johnson;P. He
设计并合成了两亲磷脂-碘化聚合物偶联物作为一种新的大分子探针，用于高度不透射线和生物相容的成像技术。聚乙二醇2000-磷脂和碘化聚酯通过点击化学的生物偶联产生了带有疏水聚酯和亲水性聚乙二醇单元体的两亲性部分，这使得它们可以自组装成囊泡或添加了囊泡。更重要的是，这些偶联物在体外X射线和细胞存活率测量中表现出高的辐射不透明度和生物相容性。这种新型生物成像造影剂的mN值为11289gmol-1，当碘当量为3.13mgmL-1时，与HEK和3T3细胞孵育48h后，在每孔20μM(20皮摩尔)的偶联物浓度下，该造影剂具有明显的X射线信号。展示了将所描述的大分子探针用于生物成像的潜力，这将进一步促进现场友好和高灵敏度的生物成像造影剂的开发，用于医疗保健诊断应用。
Amphiphilic phospholipid-iodinated polymer conjugates were designed and synthesized as new macromolecular probes for a highly radiopaque and biocompatible imaging technology. Bioconjugation of PEG 2000-phospholipids and iodinated polyesters by click chemistry created amphiphilic moieties with hydrophobic polyesters and hydrophilic PEG units, which allowed their self-assemblies into vesicles or spiked vesicles. More importantly, the conjugates exhibited high radiopacity and biocompatibility in in vitro X-ray and cell viability measurements. This new type of bioimaging contrast agent with a Mn value of 11 289 g mol-1 was found to have a significant X-ray signal at 3.13 mg mL-1 of iodine equivalent than baseline and no cytotoxicity after 48 hours incubation of with HEK and 3T3 cells at 20 μM (20 picomoles) concentration of conjugates per well. The potential of adopting the described macromolecular probes for bioimaging was demonstrated, which could further promote the development of a field-friendly and highly sensitive bioimaging contrast agent for point-of-care diagnostic applications.