Hierarchically Porous Hydroxyapatite Hybrid Scaffold Incorporated with Reduced Graphene Oxide for Rapid Bone Ingrowth and Repair
Hierarchically Porous Hydroxyapatite Hybrid Scaffold Incorporated with Reduced Graphene Oxide for Rapid Bone Ingrowth and Repair
复制标题
含有还原氧化石墨烯的分层多孔羟基磷灰石混合支架可实现骨快速生长和修复
DOI：
10.1021/acsnano.9b04723
复制
发表时间：
2019-08-01
期刊：
影响因子：
17.1
通讯作者：
Zhou, Zongke
中科院分区：
文献类型：
--
作者：
Zhou, Kai;Yu, Peng;Zhou, Zongke
羟基磷灰石（Hydroxyapatite，HA）是传统的骨组织替代材料，因其良好的生物相容性而广泛应用于临床骨缺损的治疗。但由于其加工难度大、骨诱导能力差等缺点，极大地限制了HA的应用。尽管已有许多改善材料可加工性和骨整合能力的策略，但材料的机械强度、孔隙率、细胞粘附等性能仍不能满足要求。本工作采用软模板法成功制备了具有分级孔结构、纳米表面形貌、合适的孔隙率和孔径、良好的生物力学强度的多孔支架。分级孔结构有利于细胞粘附、流体转移和细胞向内生长。此外，负载的还原氧化石墨烯（rGO）可以提高粘附性，促进骨髓间充质干细胞的增殖和自发成骨分化。然后支架被压碎、降解并被新形成的骨包裹，并且新形成的骨逐渐取代支架。支架的降解速率与新骨形成的速率很好地匹配。HA/rGO复合支架可显著促进骨长入和严重骨缺损的修复，为大段骨组织工程提供了一种潜在的临床应用材料。
Hydroxyapatite (HA), the traditional bone tissue replacement material was widely used in the clinical treatment of bone defects because of its excellent biocompatibility. However, the processing difficulty and poor osteoinductive ability greatly limit the application of HA. Although many strategies have been reported to improve the machinability and osteointegration ability, the performance including mechanical strength, porosity, cell adhesion, etc. of material still can not meet the requirements. In this work, a soft template method was developed and a porous scaffold with hierarchical pore structure, nano surface morphology, suitable porosity and pore size, and good biomechanical strength was successfully prepared. The hierarchical pore structure is beneficial for cell adhesion, fluid transfer, and cell ingrowth. Moreover, the loaded reduced graphene oxide (rGO) can improve the adhesion and promote the proliferation and spontaneous osteogenic differentiation bone marrow mesenchymal stem cells. The scaffold is then crushed, degraded and wrapped by the newly formed bone and the newly formed bone gradually replaces the scaffold. The degradation rate of the scaffold well matches the rate of the new bone formation. The hierarchical porous HA/rGO composite scaffolds can greatly accelerate the bone ingrowth in the scaffold and bone repair in critical bone defects, thus providing a clinical potential candidate for large segment bone tissue engineering.