Histone H2B Monoubiquitination Mediated by HISTONE MONOUBIQUITINATION1 and HISTONE MONOUBIQUITINATION2 Is Involved in Anther Development by Regulating Tapetum Degradation-Related Genes in Rice
Histone H2B Monoubiquitination Mediated by HISTONE MONOUBIQUITINATION1 and HISTONE MONOUBIQUITINATION2 Is Involved in Anther Development by Regulating Tapetum Degradation-Related Genes in Rice
复制标题
组蛋白单泛素化 1 和组蛋白单泛素化 2 介导的组蛋白 H2B 单泛素化通过调节水稻绒毡层降解相关基因参与花药发育
DOI：
10.1104/pp.114.256578
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发表时间：
2015-08-01
期刊：
影响因子：
7.4
通讯作者：
Liu, Yong-Xiu
中科院分区：
文献类型：
--
作者：
Cao, Hong;Li, Xiaoying;Liu, Yong-Xiu
组蛋白H_2B单素化(H_2Bub1)是真核基因转录的重要调控机制，对植物的正常发育起着至关重要的作用。然而，H_2Bub1在生殖发育中的作用仍不清楚。在此，我们报道了水稻组蛋白单体ONOUBIQUITINATION1(OsHUB1)和OsHUB2，这两个蛋白是拟南芥HUB1和HUB2的同源蛋白，它们在H_2Bub1中作为E3连接酶，参与水稻花药的后期发育。OShub突变体在减数分裂后的花药中表现出绒毡层发育异常和花粉败育。OsHUB1或OsHUB2的敲除导致H2Bub1的丢失和组蛋白3(H3K4me2)上二甲基化赖氨酸-4水平的降低。花药转录组分析表明，与绒毡层降解相关的几个关键基因OsC4、水稻半胱氨酸蛋白酶1(OsCP1)和未发育的绒毡层1(UDT1)在突变体中表达下调。进一步的染色质免疫沉淀分析表明，H_2Bub1直接靶向OsC4、OsCP1和UDT1基因，并且H_2Bub_1和H_3K4me2在靶标中的浓缩在一定程度上是一致的。我们的研究表明，由OsHUB1和OsHUB2介导的组蛋白H2 B单素化是一种重要的表观遗传修饰，与H3K4me2共同调控水稻花药发育的转录调控。
Histone H2B monoubiquitination (H2Bub1) is an important regulatory mechanism in eukaryotic gene transcription and is essential for normal plant development. However, the function of H2Bub1 in reproductive development remains elusive. Here, we report rice (Oryza sativa) HISTONE MONOUBIQUITINATION1 (OsHUB1) and OsHUB2, the homologs of Arabidopsis (Arabidopsis thaliana) HUB1 and HUB2 proteins, which function as E3 ligases in H2Bub1, are involved in late anther development in rice. oshub mutants exhibit abnormal tapetum development and aborted pollen in postmeiotic anthers. Knockout of OsHUB1 or OsHUB2 results in the loss of H2Bub1 and a reduction in the levels of dimethylated lysine-4 on histone 3 (H3K4me2). Anther transcriptome analysis revealed that several key tapetum degradation-related genes including OsC4, rice Cysteine Protease1 (OsCP1), and Undeveloped Tapetum1 (UDT1) were down-regulated in the mutants. Further, chromatin immunoprecipitation assays demonstrate that H2Bub1 directly targets OsC4, OsCP1, and UDT1 genes, and enrichment of H2Bub1 and H3K4me2 in the targets is consistent to some degree. Our studies suggest that histone H2B monoubiquitination, mediated by OsHUB1 and OsHUB2, is an important epigenetic modification that in concert with H3K4me2, modulates transcriptional regulation of anther development in rice.