SEQUENCE-ANALYSIS OF THE PORCINE EPIDEMIC DIARRHEA VIRUS GENOME BETWEEN THE NUCLEOCAPSID AND SPIKE PROTEIN GENES REVEALS A POLYMORPHIC ORF
SEQUENCE-ANALYSIS OF THE PORCINE EPIDEMIC DIARRHEA VIRUS GENOME BETWEEN THE NUCLEOCAPSID AND SPIKE PROTEIN GENES REVEALS A POLYMORPHIC ORF
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DOI：
10.1006/viro.1994.1058
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发表时间：
1994-02-01
期刊：
影响因子：
3.7
通讯作者：
LAUDE, H
中科院分区：
文献类型：
--
作者：
DUARTE, M;TOBLER, K;LAUDE, H
为进一步研究猪流行性腹泻病毒(PEDV)的基因组结构，分别构建了两株强毒株(S)核衣壳蛋白基因和棘蛋白基因之间的克隆，并对其进行了序列测定。在已鉴定的三个主要ORF中，有两个编码主要和次要冠状病毒膜蛋白M和SM。根据病毒亚基因组mRNAs的模式，在S和SM基因之间可能存在一个潜在的单一ORF，命名为ORF3。在两个分离物的ORF3中都观察到了显著的变异，本质上是由聚集在几个座位上的短缺失所产生的。预测的最大多肽为223个氨基酸，与同一基因亚组其他成员可能编码的多肽同源，包括两个较短的人类呼吸道病毒丙型肝炎病毒229E多肽和一个传染性胃肠炎病毒TGEV多肽。这一同源性表明，两个丙型肝炎病毒开放阅读框可能起源于同一前体。这些多肽的功能尚不清楚，但PEDV ORF3和相关ORF的预测产物具有提示膜相关蛋白的特征。
In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified, two were found to encode the major and minor coronavirus membrane proteins M and sM. A potentially single ORF, designated ORF3 according to the pattern of the viral subgenomic mRNAs, could be identified between the S and sM genes. A striking variability, essentially generated by short deletions clustered in a few loci, was observed in the ORF3 of both isolates. The largest predicted polypeptide of 223 amino acids showed homology with polypeptides potentially encoded by other members of the same genetic subset, including two shorter polypeptides of human respiratory virus HCV 229E and one of transmissible gastroenteritis virus TGEV. This homology suggests that the two HCV ORFs may have originated from a single precursor. The function of these polypeptides is not known, but the predicted products of the PEDV ORF3 and related ORFs share features suggestive of a membrane-associated protein.