Identification of Dof transcription factors in ramie (Boehmeria nivea L. Gaud) and their expression in response to different nitrogen treatments
Identification of Dof transcription factors in ramie (Boehmeria nivea L. Gaud) and their expression in response to different nitrogen treatments
复制标题
苎麻 (Boehmeria nivea L. Gaud) 中 Dof 转录因子的鉴定及其在不同氮处理下的表达
DOI：
10.1007/s13205-018-1512-8
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发表时间：
2018
期刊：
影响因子：
2.8
通讯作者：
Liu Touming
中科院分区：
文献类型：
--
作者：
Xu Xiaomin;Li Fu;Wang Yanzhou;Tang Shouwei;Dai Qiuzhong;Zhu Siyuan;Liu Touming
从苎麻（Boehmeria niveaL.）的基因组和转录组数据中鉴定出19个DNA单指结合（Dof）转录因子基因。Gaud）。进行了化学特征、亚核定位、基序分析、系统发育分析和组织特异性分析。为了筛选参与氮代谢的BnDof基因，我们分析了不同氮素条件（N0、N4、N8和N12）下BnDof基因在不同组织中的表达模式。结果表明，8个BnDof基因在N0和N4中表达量较高，其中BnDof07（茎）和BnDof14（根）在N0和N4中表达量最高，BnDof15（茎和叶）和BnDof18（茎）在N0中表达量最高，表明这4个基因可能参与了氮胁迫。在叶片中，BnDof01、BnDof06、BnDof09和BnDof19的表达水平在N8和N12中较高，但在N0和N4中较低。本研究结果提供了深入了解苎麻氮代谢，强调需要在未来的研究中进一步详细调查这方面。
19 DNA binding with one finger (Dof) transcription factor genes were identified from genome and transcriptome data of ramie (Boehmeria niveaL. Gaud). Chemical character, subnuclear localization, motif analysis, phylogenetic analysis, and tissue-specific analysis were performed. To selectBnDofgenes participating in nitrogen metabolism, we analyzed the expression patterns ofBnDofgenes in different nitrogen conditions (N0, N4, N8, and N12) in different tissues. As a result, eightBnDofgenes were identified.BnDof07(stem) andBnDof14(root) had higher expression levels in N0 and N4, andBnDof15(stem and leaves) andBnDof18(stem) both exhibited the highest expression level in N0, suggesting that these four genes may take part in nitrogen stress. In the leaves,BnDof01, BnDof06, BnDof09, andBnDof19expression levels were higher in N8 and N12 but lower in N0 and N4. The present findings provide insights into nitrogen metabolism in ramie highlighting the need to investigate this aspect in further detail in future studies.