A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry
A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry
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DOI：
10.1021/ac010979m
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发表时间：
2002-04-01
影响因子：
7.4
通讯作者：
Ni, JY
中科院分区：
文献类型：
--
作者：
Guo, Z;Zhang, QC;Ni, JY
我们报告了一种新的方法，称为基质抑制激光解吸/电离，以提高低质量分子的MALDI-TOF质谱分析。在该方法中，表面活性剂十六烷基三甲基溴化铵（CTAB）加入到α-氰基-4-羟基肉桂酸溶液的常规基质中，制备MALDI样品。在MALDI过程中，CTAB的存在可以基本上甚至完全抑制基质相关离子背景。因此，可以在低质量范围内常规获得非常干净的质谱。此外，CTAB的存在可以显著提高低质量分子的质量分辨率。可以看出，在基质/CTAB比为1000：1时常规获得高质量光谱。该方法已成功地用于分析各种低质量分子。
We report a novel method termed matrix suppressed laser desorption/ionization to improve the analysis of low-mass molecules by MALDI-TOF mass spectrometry. In this method, the surfactant of cetrimonium bromide (CTAB) is added to the conventional matrix of alpha-cyano-4-hydroxycinnamic acid solution to prepare the MALDI samples. During the MALDI process, the presence of CTAB could substantially or even completely suppress the matrix-related ion background. As a result, very clean mass spectra can be routinely obtained in the low-mass range. In addition, the presence of CTAB can significantly improve the mass resolution of low-mass molecules. It is seen that high-quality spectra were routinely obtained at a matrix/CTAB ratio of 1000:1. This method has been successfully used to analyze a variety of low-mass molecules.