In Vitro Bioactivity and Biocompatibility of Bio-Inspired Ti-6Al-4V Alloy Surfaces Modified by Combined Laser Micro/Nano Structuring
In Vitro Bioactivity and Biocompatibility of Bio-Inspired Ti-6Al-4V Alloy Surfaces Modified by Combined Laser Micro/Nano Structuring
复制标题
激光微/纳米组合改性仿生 Ti-6Al-4V 合金表面的体外生物活性和生物相容性
DOI：
10.3390/molecules25071494
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发表时间：
2020-04-01
期刊：
影响因子：
4.6
通讯作者：
Cheng, Guanghua
中科院分区：
文献类型：
--
作者：
Li, Chen;Yang, Yong;Cheng, Guanghua
生物活性和生物相容性在牙科和骨科植入物的成功中起着关键作用。尽管大多数商业种植体系统使用各种表面微结构，但能够控制骨整合的理想多尺度形貌尚未产生结论性结果。受树蛙趾垫皮肤结构的各向同性粘附性和Morpho蝴蝶翅膀鳞片上波纹状脊的各向异性粘附性的启发，分别采用微秒激光直写和飞秒激光诱导周期性表面结构在钛合金植入体上制备了微六边形阵列和定向纳米波纹等复合微/纳米结构，以提高细胞粘附性，植入物上的排列和增殖。分析了Ti-6Al-4V表面光滑、微六边形和微纳米复合结构试样在模拟体液中的生物活性和成骨细胞MC 3 T3增殖的生物相容性的主要差异。值得注意的是，仿生微/纳米结构在体外实验后显示出最好的生物活性和生物相容性，同时，纳米波纹能够诱导微六边形内的细胞排列。这些差异的原因被发现在地形线索。提出了一种利用复合微/纳米结构控制钛合金植入物骨整合的创新功能化策略，这在各种再生医学应用和植入领域具有重要意义。
The bioactivity and biocompatibility play key roles in the success of dental and orthopaedic implants. Although most commercial implant systems use various surface microstructures, the ideal multi-scale topographies capable of controlling osteointegration have not yielded conclusive results. Inspired by both the isotropic adhesion of the skin structures in tree frog toe pads and the anisotropic adhesion of the corrugated ridges on the scales of Morpho butterfly wings, composite micro/nano-structures, including the array of micro-hexagons and oriented nano-ripples on titanium alloy implants, were respectively fabricated by microsecond laser direct writing and femtosecond laser-induced periodic surface structures, to improve cell adherence, alignment and proliferation on implants. The main differences in both the bioactivity in simulated body fluid and the biocompatibility in osteoblastic cell MC3T3 proliferation were measured and analyzed among Ti-6Al-4V samples with smooth surface, micro-hexagons and composite micro/nano-structures, respectively. Of note, bioinspired micro/nano-structures displayed the best bioactivity and biocompatibility after in vitro experiments, and meanwhile, the nano-ripples were able to induce cellular alignment within the micro-hexagons. The reasons for these differences were found in the topographical cues. An innovative functionalization strategy of controlling the osteointegration on titanium alloy implants is proposed using the composite micro/nano-structures, which is meaningful in various regenerative medicine applications and implant fields.