The vaginal metabolome and microbiota of cervical HPV-positive and HPV-negative women: a cross-sectional analysis
The vaginal metabolome and microbiota of cervical HPV-positive and HPV-negative women: a cross-sectional analysis
复制标题
DOI：
10.1111/1471-0528.15981
复制
发表时间：
2019-11-20
影响因子：
5.8
通讯作者：
Brotman, R. M.
中科院分区：
文献类型：
--
作者：
Borgogna, J. C.;Shardell, M. D.;Brotman, R. M.
目的研究宫颈HPV感染与未感染妇女阴道代谢物组的特征。设计横截面。马里兰州大学公共卫生学院健康行为研究中心。样本39名参与者，13名被归类为HPV阴性，26名被归类为HPV阳性（任何基因型; HPV+），其中14名至少有一种高危HPV毒株（hrHPV）阳性。方法采用16 S rRNA基因扩增产物测序法、气相色谱-质谱联用技术和液相色谱-质谱联用技术，分析阴道中段拭子中的细菌组成和37种HPV DNA。主要结果测量代谢物丰度。结果阴道微生物群聚类为群落状态类型（CST）I（卷曲乳杆菌为主）、CST III（惰性乳杆菌为主）和CST IV（低乳杆菌，“分子-BV”）。在校正CST和吸烟后，HPV+女性的生物胺和磷脂浓度高于HPV-女性。HPV+和HPV-女性的代谢组学特征在CST分层中不同。在CST III中，HPV+女性的生物胺和糖原相关代谢物浓度高于HPV-女性。在CST IV中，HPV+受试者中谷胱甘肽、糖原和磷脂相关代谢物的浓度低于HPV（-）受试者。在所有的CST中，与只有低风险HPV（lrHPV）的女性相比，hrHPV毒株的女性具有较低的氨基酸，脂质和肽浓度。结论HPV阳性妇女的阴道代谢组与HPV阴性妇女的代谢组在几种代谢物方面不同，包括生物胺、谷胱甘肽和脂质相关代谢物。如果在未来的研究中证实还原型谷胱甘肽和氧化型谷胱甘肽水平升高与HPV发病率/持续性之间的时间关系，则抗氧化治疗可被视为非手术HPV控制干预。代谢组学研究：HPV阳性女性的阴道微环境可能为非手术干预提供信息。
Objective Characterise the vaginal metabolome of cervical HPV-infected and uninfected women. Design Cross-sectional. Setting The Center for Health Behavior Research at the University of Maryland School of Public Health. Sample Thirty-nine participants, 13 categorised as HPV-negative and 26 as HPV-positive (any genotype; HPV+), 14 of whom were positive with at least one high-risk HPV strain (hrHPV). Method Self-collected mid-vaginal swabs were profiled for bacterial composition by 16S rRNA gene amplicon sequencing, metabolites by both gas and liquid chromatography mass spectrometry, and 37 types of HPV DNA. Main outcome measures Metabolite abundances. Results Vaginal microbiota clustered into Community State Type (CST) I (Lactobacillus crispatus-dominated), CST III (Lactobacillus iners-dominated), and CST IV (low-Lactobacillus, 'molecular-BV'). HPV+ women had higher biogenic amine and phospholipid concentrations compared with HPV- women after adjustment for CST and cigarette smoking. Metabolomic profiles of HPV+ and HPV- women differed in strata of CST. In CST III, there were higher concentrations of biogenic amines and glycogen-related metabolites in HPV+ women than in HPV- women. In CST IV, there were lower concentrations of glutathione, glycogen, and phospholipid-related metabolites in HPV+ participants than in HPV(- )participants. Across all CSTs, women with hrHPV strains had lower concentrations of amino acids, lipids, and peptides compared with women who had only low-risk HPV (lrHPV). Conclusions The vaginal metabolome of HPV+ women differed from HPV- women in terms of several metabolites, including biogenic amines, glutathione, and lipid-related metabolites. If the temporal relation between increased levels of reduced glutathione and oxidised glutathione and HPV incidence/persistence is confirmed in future studies, anti-oxidant therapies may be considered as a non-surgical HPV control intervention. Tweetable abstract Metabolomics study: Vaginal microenvironment of HPV+ women may be informative for non-surgical interventions.