Novel biocatalysts by identification and design
Novel biocatalysts by identification and design
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DOI：
10.1080/10242420410001710056
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发表时间：
2004-03-01
影响因子：
1.8
通讯作者：
Jaeger, KE
中科院分区：
文献类型：
--
作者：
Eggert, T;Leggewie, C;Jaeger, KE
由细菌和真核生物产生的酶目前被用于多种不同的生物技术应用。对新颖且通常非天然底物具有活性的酶的需求迅速增加，引发了酶分离和设计的新颖分子生物学方法的发展。宏基因组方法是一种独立于培养的方法，可以直接克隆和表达环境 DNA，从而提供大量迄今为止未知的生物催化剂。此外，新发现的或现有的生物催化剂可以通过不同的定向进化方法进一步优化。在这里，概述了宏基因组方法的原理，并提出了结合合理设计和定向进化来优化细菌脂肪酶的策略。
Enzymes produced from bacteria and eukaryotic organisms are presently being used for a large variety of different biotechnological applications. The rapidly increasing demand for enzymes which are active towards novel and often non-natural substrates has triggered the development of novel molecular biological methods of enzyme isolation and design. The metagenome approach is a cultivation-independent method which allows the direct cloning and expression of environmental DNA thereby providing access to a wealth of so-far unknown biocatalysts. Additionally, newly identified or existing biocatalysts can be further optimized by different methods of directed evolution. Here, the principle of the metagenome approach is outlined and a strategy is presented for the optimization of a bacterial lipase using a combination of rational design and directed evolution.