In Situ Observation of Dislocations in Protein Crystals during Growth by Advanced Optical Microscopy
In Situ Observation of Dislocations in Protein Crystals during Growth by Advanced Optical Microscopy
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DOI：
10.1021/cg049605n
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发表时间：
2005-08
影响因子：
3.8
通讯作者：
G. Sazaki;K. Tsukamoto;Satomi Yai;M. Okada;K. Nakajima
中科院分区：
文献类型：
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作者：
G. Sazaki;K. Tsukamoto;Satomi Yai;M. Okada;K. Nakajima
我们尝试用激光共聚焦显微镜结合差示干涉对比显微镜（LCM-DIM）原位观察四方溶菌酶晶体中的缺陷。双折射显微镜（BM）和相衬显微镜（PCM）也被用于原位观察。LCM-DIM使我们第一次能够观察到晶体的{11 10}表面，并在生长过程中看到与光束垂直的位错周围的应变场。使用BM和PCM，在生长过程中还可以看到晶体内部较大的夹杂物（60 ~ 300 μm）。我们发现位错呈束状出现，可能产生于晶体内部较大夹杂物的外围。通过刻蚀实验证实了位错的存在，在刻蚀实验中，我们观察到两种类型的刻蚀坑：点底和平底；前者对应于晶体内部的位错，而后者对应于…
We attempted to visualize defects in a tetragonal lysozyme crystal in situ by laser confocal microscopy combined with differential interference contrast microscopy (LCM-DIM). Birefringence microscopy (BM) and phase-contrast microscopy (PCM) were also employed for the in situ observations. LCM-DIM enabled us to observe the {1 1 0} surfaces of the crystals and visualize the strain fields around the dislocations normal to a light beam with sufficient contrast during growth for the first time. Relatively large inclusions (60 to 300 μm) inside the crystal could also be visualized during growth, with the use of BM and PCM. We found that the dislocations appeared in bundles and were probably generated at the periphery of the relatively large inclusions inside the crystal. The existence of the dislocations was confirmed by etching experiments, in which we observed two kinds of etch pits: those with a point bottom and those with a flat bottom; the former corresponded to the dislocations inside the crystal and the...