Determination of iridoid glucosides for quality assessment of Herba Oldenlandiae by high-performance liquid chromatography
Determination of iridoid glucosides for quality assessment of Herba Oldenlandiae by high-performance liquid chromatography
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DOI：
10.1248/cpb.54.1131
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发表时间：
2006-08-01
影响因子：
1.7
通讯作者：
Zhao, Zhong-Zhen
中科院分区：
文献类型：
--
作者：
Liang, Zhi-Tao;Jiang, Zhi-Hong;Zhao, Zhong-Zhen
白花蛇舌草（Herba Oldenlandia diffusa（WILLD.）RoxB.（茜草科），已正式列入《中国药典》。在草药市场上，有两种替代品来源于O。corymbosa（L.）LAM和O.细花通常使用。因此，建立一种适合于白花蛇舌草的质量评价方法是当务之急。本文建立了一种简便、可靠的测定白花蛇舌草中草精苷（1）、E-6-O-p-香豆酰斯堪的纳维亚苷甲酯（2）和E-6-O-p-香豆酰斯堪的纳维亚苷甲酯-10-甲醚（3）含量的高效液相色谱法。弥漫的。其中，化合物3为从O.弥漫的。这些化合物均为首次用于白花蛇舌草的质量评价。结果表明，化合物1-3在不同样品中的含量差异较大，而化合物2和3在O的两种替代物中的含量较低。弥漫的。该方法适用于白花蛇舌草的质量控制，可用于鉴别其易混淆品种。该方法已得到充分验证，线性、准确度、精密度和稳定性均符合要求。
Herba Oldenlandiae, the dried herb of Oldenlandia diffusa (WILLD.) RoxB. (Family Rubiaceae), is officially listed in the Chinese Pharmacopoeia. In the herbal market, two substitutes originated from O. corymbosa (L.) LAM and O. tenelliflora BL. are commonly used. In light of this, the target in setting up a method for quality assessment of Herba Oldenlandiae is urgently needed. In this article, a simple and reliable high-performance liquid chromatographic (HPLC) method was developed for quantifying asperuloside (1), E-6-O-p-coumaroyl scandoside methyl ester (2) and E-6-O-p-coumaroyl scandoside methyl ester-10-methyl ether (3) in Herba Oldenlandiae derived from O. diffusa. Among them, compound 3 is a new compound isolated from O. diffusa. All these unique compounds were used as markers for the first time in the quality assessment of Herba Oldeniandiae. The results showed that the contents of compounds 1-3 were significantly varied among different samples whilst those of compounds 2 and 3 were found to be lower in contents in the two substitutes of O. diffusa. The analytical method is suitable for quality control of Herba Oldenlandiae and useful in differentiation from its confusable species. The method has been fully validated with satisfactory linearity, accuracy, precision and stability.