Circulating Tumor Cells Were Associated with the Number of T Lymphocyte Subsets and NK Cells in Peripheral Blood in Advanced Non-Small-Cell Lung Cancer.
Circulating Tumor Cells Were Associated with the Number of T Lymphocyte Subsets and NK Cells in Peripheral Blood in Advanced Non-Small-Cell Lung Cancer.
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晚期非小细胞肺癌中循环肿瘤细胞与外周血中 T 淋巴细胞亚群和 NK 细胞的数量相关
DOI：
10.1155/2017/5727815
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Ye L;Zhang F;Li H;Yang L;Lv T;Gu W;Song Y
在本研究中，我们旨在探讨非小细胞肺癌（NSCLC）外周血中循环肿瘤细胞（CTCs）与T淋巴细胞亚群以及自然杀伤（NK）细胞之间的相关性。外周血CTCs通过SET - iFISH技术测定，流式细胞术用于测定T淋巴细胞亚群和NK细胞的分布。41例（49%）患者CTCs呈阳性。逻辑回归分析显示，在Ⅳ期患者中，CTCs数量与CD3⁺、CD4⁺、CD4⁺/CD8⁺的比值以及NK细胞百分比呈负相关，而在这些患者中，CTCs数量与调节性T细胞（Tregs）比值呈正相关。结合临床病理资料进行多因素分析以确定CTCs阳性的危险因素。分化程度、NSCLC分期、CD3⁺CD4⁺细胞百分比、Tregs以及NK细胞是CTCs的独立危险因素。CTCs与NSCLC患者外周血免疫监视功能降低有关。免疫监视功能的降低促使CTCs逃避免疫细胞的杀伤作用，并导致靶器官转移病灶的形成。
In this study, we aim to investigate the correlation between circulating tumor cells (CTCs) and the T lymphocyte subsets and NK cells in peripheral blood in non-small-cell lung cancer (NSCLC). The peripheral blood CTCs were determined by SET-iFISH. Flow cytometry was used to determine the distribution of T lymphocyte subsets and NK cells. Forty-one (49%) patients showed positivity for CTCs. Logistic regression analysis revealed CTC number was negatively correlated with the ratio of CD3+, CD4+, CD4+/CD8+, and NK % in patients at stage IV, while in a positive correlation was noticed between CTC number and regulatory T cell (Tregs) ratio in these patients. Multivariate analysis was performed in combination with the clinical-pathological materials to identify the risk factors for CTC positivity. Differentiation, NSCLC stage, percentages of CD3+CD4+ cells, Tregs, and NK cells were the independent risk factors for CTCs. CTCs were associated with the decrease of immune surveillance in the peripheral blood in NSCLC patients. The decrease of immune surveillance contributed to the escape of CTCs from the killing effects of the immunocytes, as well as the formation of metastasized lesions in the target organs.
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影响因子：
13.6
作者：
Ohaegbulam KC;Assal A;Lazar-Molnar E;Yao Y;Zang X
通讯作者：
Zang X
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0.9
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He AR
影响因子：
64.8
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Mellman, Ira;Coukos, George;Dranoff, Glenn
通讯作者：
Dranoff, Glenn
影响因子：
3.9
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Mego M;Gao H;Cohen EN;Anfossi S;Giordano A;Sanda T;Fouad TM;De Giorgi U;Giuliano M;Woodward WA;Alvarez RH;Valero V;Ueno NT;Hortobagyi GN;Cristofanilli M;Reuben JM
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