The comprehensive assessment of local immune status of ovarian cancer by the clustering of multiple immune factors
The comprehensive assessment of local immune status of ovarian cancer by the clustering of multiple immune factors
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DOI：
10.1016/j.clim.2011.08.013
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发表时间：
2011-12-01
影响因子：
8.6
通讯作者：
Konishi, Ikuo
中科院分区：
文献类型：
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作者：
Hamanishi, Junzo;Mandai, Masaki;Konishi, Ikuo
本研究旨在通过对卵巢癌患者肿瘤浸润免疫细胞和免疫抑制因子的综合分析，评价卵巢癌患者局部免疫状态，阐明局部免疫在临床过程中的作用。采用免疫组化法检测70例卵巢癌组织中CD 1 α +、CD 4+、CD 8+、CD 57+、叉头盒P3+和程序性细胞死亡1+细胞数，并检测免疫抑制因子程序性细胞死亡1配体（PD-L）1、PD-L 2、环氧化酶（考克斯）1、考克斯2和转化生长因子β 1的表达。然后对这些参数进行层次聚类，显示这四个聚类为卵巢癌病例。第1组的预后明显优于其他组，其特征是CD 4+和CD 8+细胞的高度浸润。总之，对局部免疫状态的综合分析导致将卵巢癌细分为具有更好或更差的预后的组，并且可以指导对局部免疫的准确理解。(C)2011 Elsevier Inc. All rights reserved.
The aim of this study was to evaluate the local immune status of human ovarian cancers by the comprehensive analysis of tumor-infiltrating immune cells and immunosuppressive factors, and to elucidate the local immunity in clinical course. The numbers of CD1 alpha+, CD4+, CD8+, CD57+, forkhead box P3+ and programmed cell death-1+ cells were counted, and the intensity of immunosuppressive factors, such as programmed cell death-1 ligand (PD-L)1, PD-L2, cyclooxygenase (COX)-1, COX-2 and transforming growth factor beta 1, were evaluated in 70 ovarian cancer specimens stained by immunohistochemistry. Then hierarchical clustering of these parameters showed the four clusters into ovarian cancer cases. Cluster 1, which had significantly better prognosis than the others, was characterized by high infiltration of CD4+ and CD8+ cells. In conclusion the comprehensive analysis of local immune status led to subdivide ovarian cancers into groups with better or worse prognoses and may guide precise understanding of the local immunity. (C) 2011 Elsevier Inc. All rights reserved.