NOVEL METHOD FOR SELECTIVE ISOLATION OF ACTINOMYCETES
NOVEL METHOD FOR SELECTIVE ISOLATION OF ACTINOMYCETES
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DOI：
10.1128/aem.46.4.925-929.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
CHRISTENSEN, DL
中科院分区：
文献类型：
--
作者：
HIRSCH, CF;CHRISTENSEN, DL
本文介绍了一种从天然混合微生物群中选择性分离放线菌的新技术。在营养琼脂上覆盖0.22-0.45µm的纤维素酯膜过滤器，并在过滤器的表面接种。在培养过程中，放线菌的分枝菌丝体穿过滤孔进入琼脂底物，而非放线菌的生长仅限于滤膜表面。去掉膜过滤器，重新培养琼脂培养基，以允许分离的放线菌菌落的发展。该程序根据放线菌特有的菌丝生长方式选择放线菌，提供了一种选择性分离的通用方法，不依赖于特殊营养介质或抗菌抗生素的使用。
A new technique for the selective isolation of actinomycetes from natural mixed microbial populations is described. A nutrient agar medium was overlaid with a 0.22-0.45 .mu.m pore cellulose ester membrane filter and the surface of the filter was inoculated. During incubation, the branched mycelia of the actinomycetes penetrated the filter pores to the underlying agar medium, whereas growth of non-actinomycete bacteria was restricted to the filter surface. The membrane filter was removed, and the agar medium was reincubated to allow the development of the isolated actinomycete colonies. This procedure selects actinomycetes on the basis of their characteristic mycelial mode of growth, offers a general method for their selective isolation and does not rely on the use of special nutrient media or of antibacterial antibiotics.