Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion.
Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion.
复制标题
通过影响细胞粘附的单克隆抗体的神经rest迁移的改变。
DOI：
10.1083/jcb.101.2.610
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发表时间：
1985-08
期刊：
影响因子：
--
通讯作者：
Bronner-Fraser M
中科院分区：
文献类型：
--
作者：
Bronner-Fraser M
使用单克隆抗体JG 22检查140-kD细胞表面复合物在神经嵴粘附和迁移中的可能作用，该单克隆抗体JG 22首先由Greve和Gottlieb（1982，J. Cell. 18：221- 229）。在体外向神经嵴细胞中加入JG 22引起铺在纤连蛋白或层粘连蛋白底物上的细胞形态的快速变化。细胞变圆并相亮，通常从培养皿中分离或形成圆形细胞的聚集体。其他组织如体节、脊索和神经管在体外不受抗体影响，即使在肌节、神经管和脊索表面的胚胎组织切片中可检测到JG 22抗原。通过一种新的微扰方法，将抗体和杂交瘤细胞显微注射到神经嵴通路上，研究了JG 22对体内神经嵴迁移的影响。杂交细胞用荧光细胞标记物标记，该标记物是无害的，并且在固定和组织切片后保存。JG 22抗体和杂交瘤细胞引起颅神经嵴迁移的显著减少，神经嵴细胞在神经管腔内的积聚，以及沿沿着异常途径的一些迁移。躯干神经嵴迁移受到的影响要小得多。在颅区和躯干区，在神经嵴细胞和JG 22杂交瘤细胞之间通常观察到一个或多个细胞直径的无细胞区。另外两种单克隆抗体1-B和1-N用作对照。1-B和1-N均与JG 22沉淀的140-kD复合物的条带结合。对照抗体均不影响体外神经嵴粘附或原位神经嵴迁移。这表明，观察到的神经嵴迁移的改变是由于140 kD复合物的功能块。
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221- 229). The addition of JG22 to neural crest cells in vitro caused a rapid change in morphology of cells plated on either fibronectin or laminin substrates. The cells became round and phase bright, often detaching from the dish or forming aggregates of rounded cells. Other tissues such as somites, notochords, and neural tubes were unaffected by the antibody in vitro even though the JG22 antigen is detectable in embryonic tissue sections on the surface of the myotome, neural tube, and notochord. The effects of the JG22 on neural crest migration in vivo were examined by a new perturbation approach in which both the antibody and the hybridoma cells were microinjected onto neural crest pathways. Hybridoma cells were labeled with a fluorescent cell marker that is nondeleterious and that is preserved after fixation and tissue sectioning. The JG22 antibody and hybridoma cells caused a marked reduction in cranial neural crest migration, a build-up of neural crest cells within the lumen of the neural tube, and some migration along aberrant pathways. Neural crest migration in the trunk was affected to a much lesser extent. In both cranial and trunk regions, a cell free zone of one or more cell diameters was generally observed between neural crest cells and the JG22 hybridoma cells. Two other monoclonal antibodies, 1-B and 1-N, were used as controls. Both 1-B and 1-N bind to bands of the 140-kD complex precipitated by JG22. Neither control antibody affected neural crest adhesion in vitro or neural crest migration in situ. This suggests that the observed alterations in neural crest migration are due to a functional block of the 140-kD complex.