Rational Design of Esterase-Insensitive Fluorogenic Probes for In Vivo Imaging.
Rational Design of Esterase-Insensitive Fluorogenic Probes for In Vivo Imaging.
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用于体内成像的酯酶不敏感荧光探针的合理设计。
DOI：
10.1021/acssensors.3c00264
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发表时间：
2023-05
期刊：
影响因子：
8.9
通讯作者：
W. Guo;Yi-Xuan Fu;Long-Can Mei;Zhao Chen;Zi-Ye Zhang;Fan Wang;Wenchao Yang;Guozhen Liu
中科院分区：
文献类型：
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作者：
W. Guo;Yi-Xuan Fu;Long-Can Mei;Zhao Chen;Zi-Ye Zhang;Fan Wang;Wenchao Yang;Guozhen Liu
Small-molecule fluorogenic probes are indispensable tools for performing research in biomedical fields and chemical biology. Although numerous cleavable fluorogenic probes have been developed to investigate various bioanalytes, few of them meet the baseline requirements for in vivo biosensing for disease diagnosis due to their insufficient specificity resulted from the remarkable esterase interferences. To address this critical issue, we developed a general approach called fragment-based fluorogenic probe discovery (FBFPD) to design esterase-insensitive probes for in vitro and in vivo applications. With the designed esterase-insensitive fluorogenic probe, we successfully achieved light-up in vivo imaging and quantitative analysis of cysteine. This strategy was further extended to design highly specific fluorogenic probes for other representative targets, sulfites, and chymotrypsin. The present study expands the bioanalytical toolboxes available and offers a promising platform to develop esterase-insensitive cleavable fluorogenic probes for in vivo biosensing and bioimaging for the early diagnosis of diseases.
