QUANTITATIVE CAPILLARY TOPOGRAPHY AND BLOOD-FLOW IN THE CEREBRAL-CORTEX OF CATS - AN INVIVO MICROSCOPIC STUDY
QUANTITATIVE CAPILLARY TOPOGRAPHY AND BLOOD-FLOW IN THE CEREBRAL-CORTEX OF CATS - AN INVIVO MICROSCOPIC STUDY
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DOI：
10.1016/0006-8993(81)90619-3
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
BING, RJ
中科院分区：
文献类型：
--
作者：
PAWLIK, G;RACKL, A;BING, RJ
在50只麻醉猫上，用显微透照法观察大脑皮层中层和深层微循环，并用高速电影摄影法记录。在一系列实验中验证了该制剂的可行性，这些实验证明了软脑膜小动脉和小动脉的自发血管运动和对化学刺激的反应性。在一个比较大的组织体积的毛细血管的投影图像的定量分析的体视学方法，以确定不对称的，高度曲折的皮质内毛细血管网络的形态和地形参数。毛细管直径（5.1 . ±. 0.84μ m）、曲率半径（中值57 μ m）、每个组织体积的总毛细血管长度（939 ± 0.5 μ m）。338.2 mm/mm 3）、毛细管体积分数（2.1 . ±. 0.51%），每组织体积的总毛细血管表面积（15.3 ± 0.51%）。4.85 mm 2/mm 3）和毛细血管间距（中值24.2 μ m）显示出显著的区域间差异。毛细管段长度的频率分布（中值108 μ m）最好用威布尔分布描述。平均90%的毛细血管连续灌注。毛细血管红细胞流（中位速度1500 μ m/s）主要是单向的并且明显不规则。毛细血管红细胞流速（CRCV）与毛细血管迂曲度呈显著正相关（r = 0.48）。极值分布最好地描述了观察到的CRCV的频率分布。流动不规则性代表白色噪声和在40-90 Hz之间的频率下的显著随机周期性。
In 50 anesthetized cats the microcirculation in intermediate and deeper layers of the cerebral cortex was visualized in vivo by microtransillumination and documented by high-speed microcinephotography. The viability of the preparation was verified in a series of experiments demonstrating spontaneous vasomotion and responsiveness to chemical stimulation of pial arterioles and small arteries. Stereological methods for quantitative analysis of projected images of capillaries in a comparatively large tissue volume were employed to determine morphometric and topographical parameters of the asymmetric, highly tortuous intracortical capillary network. Capillary diameters (5.1 .+-. 0.84 .mu.m), radii of curvature (median 57 .mu.m), total capillary lengths per tissue volume (939 .+-. 338.2 mm/mm3), capillary volume fractions (2.1 .+-. 0.51%), total capillary surface areas per tissue volume (15.3 .+-. 4.85 mm2/mm3) and intercapillary distances (median 24.2 .mu.m) showed significant interregional differences. The frequency distribution of the lengths of capillary segments (median 108 .mu.m) was best described by a Weibull distribution. An average 90% of all capillaries were continuously perfused. Capillary red cell flow (median velocity 1500 .mu.m/s) was predominantly unidirectional and conspicuously irregular. The variance of capillary red cell velocities (CRCV) was significantly correlated (r = 0.48) with capillary tortuosity. An extreme value distribution best described the observed frequency distribution of CRCV. Flow irregularities represented white noise and a significant stochastic periodicity at frequencies between 40-90 Hz.