Novel genes derived from noncoding DNA in Drosophila melanogaster are frequently X-linked and exhibit testis-biased expression
Novel genes derived from noncoding DNA in Drosophila melanogaster are frequently X-linked and exhibit testis-biased expression
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DOI：
10.1073/pnas.0509809103
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发表时间：
2006-06-27
影响因子：
11.1
通讯作者：
Begun, David J.
中科院分区：
文献类型：
--
作者：
Levine, Mia T.;Jones, Corbin D.;Begun, David J.
对最近进化的基因的描述表明了几种起源机制，包括外显子改组、基因分裂/融合、反转录转座、复制-趋异和横向基因转移，所有这些都涉及将先前存在的基因或遗传元件募集到新的功能中。非编码DNA在新基因起源中的重要性仍然是一个悬而未决的问题。我们利用基因模型系统Drosophilamelanogaster的基因组和相关物种的基因组序列进行了全基因组搜索，以寻找新的D。来自非编码DNA的黑腹基因。在这里，我们描述了五个这样的基因，其中四个是X连锁的。我们的RT-PCR实验表明，所有五个推定的新基因主要在睾丸中表达。这些数据支持这样的观点，即这些新的基因来自祖先的非编码序列，新的，有利的基因很可能会在与男性生殖有关的选择压力下入侵种群。
Descriptions of recently evolved genes suggest several mechanisms of origin including exon shuffling, gene fission/fusion, retrotransposition, duplication-divergence, and lateral gene transfer, all of which involve recruitment of preexisting genes or genetic elements into new function. The importance of noncoding DNA in the origin of novel genes remains an open question. We used the well annotated genome of the genetic model system Drosophila melanogaster and genome sequences of related species to carry out a whole-genome search for new D. melanogaster genes that are derived from noncoding DNA. Here, we describe five such genes, four of which are X-linked. Our RT-PCR experiments show that all five putative novel genes are expressed predominantly in testes. These data support the idea that these novel genes are derived from ancestral noncoding sequence and that new, favored genes are likely to invade populations under selective pressures relating to male reproduction.