Synthesis and evaluation of a silica-bonded concanavalin A material for lectin affinity enrichment of N-linked glycoproteins and glycopeptides
Synthesis and evaluation of a silica-bonded concanavalin A material for lectin affinity enrichment of N-linked glycoproteins and glycopeptides
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DOI：
10.1039/c4ay02286f
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发表时间：
2015
影响因子：
3.1
通讯作者：
Liu Yujie;D. Fu;Yuan-sheng Xiao;Zhimou Guo;Long Yu;Xinmiao Liang
中科院分区：
文献类型：
--
作者：
Liu Yujie;D. Fu;Yuan-sheng Xiao;Zhimou Guo;Long Yu;Xinmiao Liang
通过一种快速、简单、稳健的方法，将伴刀豆球蛋白A与硅胶共价键合，合成了一种凝集素亲和层析材料（命名为SG Con A）。该材料表现出高选择性和效率，以及低样品损失的选择性富集的N-连接的糖蛋白和糖肽。
A lectin affinity chromatography material was synthesized by covalent bonding of concanavalin A onto silica gel (named SG Con A) via a rapid, simple and robust method. The material exhibited high selectivity and efficiency as well as low sample loss for the selective enrichment of N-linked glycoproteins and glycopeptides.