Ontogeny of gonadotropin receptors in the fetal and neonatal rat testis.
Ontogeny of gonadotropin receptors in the fetal and neonatal rat testis.
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DOI：
10.1210/endo-114-2-470
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发表时间：
1984-02
期刊：
影响因子：
4.8
通讯作者：
D. Warren;I. Huhtaniemi;J. Tapanainen;M. Dufau;K. Catt
中科院分区：
文献类型：
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作者：
D. Warren;I. Huhtaniemi;J. Tapanainen;M. Dufau;K. Catt
为了评估LH受体可能参与调节胎儿和新生大鼠睾丸睾酮产生的程度，测定了LH受体的性腺含量，并将其与性分化和新生儿早期每天间隔的睾酮和其他类固醇的性腺含量相关联。此外，为了确定FSH在胎儿生命期间是否具有潜在作用，在妊娠14.5天至21.5天（出生前）测量了FSH受体。在妊娠15.5天时，首次在胎鼠睾丸中检测到LH受体，浓度为0.072 +/- 0.016 fmol/性腺。这是以前的研究表明对LH刺激的生物学反应，cAMP和睾酮产生增加的时间。LH受体含量从妊娠15.5-17.5天保持恒定，然后在妊娠18.5天急剧增加至0.706 +/- 0.046 fmol/性腺，与胎儿睾丸睾酮含量激增一致。LH受体含量持续上升，直到出生，在妊娠21.5天达到最高水平2.00 +/- 0.24 fmol/性腺，并没有显着差异，从这个值到出生后5天，而睾丸内睾酮含量出生后下降。FSH受体可以从妊娠17.5天开始测量，但在胎儿生命的19.5天内保持非常低的水平。在20.5天，FSH受体水平开始急剧上升，并持续增加到妊娠21.5天，就在出生前。这些数据表明，当胎儿睾丸对LH刺激产生反应时，LH受体是可测量的。LH受体的增加可能作为一种机制，放大了低循环LH水平对胎儿睾丸的刺激作用，同时伴随着睾丸内睾酮的增加。出生后睾酮产生的减少不能归因于新生儿性腺中LH受体的减少。由于出生后5 α-还原雄激素增加，其他可能性，如替代类固醇途径，是睾丸激素含量下降的更可能解释。胎儿睾丸中FSH受体的存在意味着这种激素可能在性腺发育中起作用，特别是在胎儿生命的最后2天。
To evaluate the extent to which LH receptors may participate in the regulation of testosterone production by the fetal and neonatal rat testis, the gonadal content of LH receptors was determined and correlated with the gonadal content of testosterone and other steroids at daily intervals throughout the period of sexual differentiation and early neonatal life. Also, to determine if FSH has a potential role during fetal life, FSH receptors were measured from 14.5 until 21.5 days of gestation, just before birth. LH receptors were first detected in the fetal rat testis at 15.5 days of gestation, at a concentration of 0.072 +/- 0.016 fmol/gonad. This is the time at which previous studies have shown a biological response to LH stimulation with increases in cAMP and testosterone production. LH receptor content remained constant from 15.5-17.5 days of gestation and then increased sharply at 18.5 days of gestation to 0.706 +/- 0.046 fmol/gonad, coincident with a surge in the testosterone content of the fetal testis. LH receptor content continued to rise until birth, reaching a maximum level of 2.00 +/- 0.24 fmol/gonad at 21.5 days of gestation, and did not significantly differ from this value through 5 days after birth, whereas intratesticular testosterone content decreased after birth. FSH receptors could be measured from 17.5 days of gestation, but remained very low through 19.5 days of fetal life. At 20.5 days, FSH receptor levels began to rise sharply and continued to increase through 21.5 days of gestation, just before birth. These data show that LH receptors are measurable at the time when the fetal testis becomes responsive to LH stimulation. It is possible that the increase in LH receptors may act as a mechanism for amplification of the stimulatory effect of low circulating LH levels on the fetal testis being concomitant with the intratesticular testosterone increase at the same stage. The decrease in testosterone production after birth cannot be attributed to a decrease in LH receptors in the neonatal gonad. Since 5 alpha-reduced androgens increase after birth, other possibilities, such as alternative steroid pathways, are a more likely explanation for the decrease in testosterone content. The presence of FSH receptors in the fetal testis imply that this hormone may have a function in the developing gonad, particularly in the last 2 days of fetal life.