Regulation of OsGRF4 by OsmiR396 controls grain size and yield in rice
Regulation of OsGRF4 by OsmiR396 controls grain size and yield in rice
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OsmiR396 对 OsGRF4 的调节控制水稻的籽粒大小和产量
DOI：
10.1038/nplants.2015.203
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发表时间：
2016-01-01
期刊：
影响因子：
18
通讯作者：
Li, Yunhai
中科院分区：
文献类型：
--
作者：
Duan, Penggen;Ni, Shen;Li, Yunhai
提高粮食产量是现代农业的关键。籽粒大小是水稻产量的关键因素之一，受数量性状基因座（QTL）调控2，3。探索新的籽粒大小QTL将有助于育种者开发出高产的优质水稻品种3，4。本研究报道了一个新的水稻粒重和粒重的半显性QTL（GS 2），该QTL编码转录因子OsGRF 4（Growth-Regulating Factor 4），受OsmiR 396调控。我们证明，GS 2中的2 bp置换突变扰乱了OsmiR 396对GS 2的定向调控，导致大而重的籽粒，并提高了籽粒产量。进一步的结果表明，GS 2与转录辅激活子OsGIF 1/2/3相互作用，OsGIF 1的过表达增加了水稻的颗粒大小和重量。因此，我们的研究结果定义了GS 2、OsGIF和OsmiR 396在控制籽粒大小和重量中的调控机制，表明该途径可用于提高作物产量。
An increase in grain yield is crucial for modern agriculture 1. Grain size is one of the key components of grain yield in rice and is regulated by quantitative trait loci (QTLs) 2, 3. Exploring new QTLs for grain size will help breeders develop elite rice varieties with higher yields 3, 4. Here, we report a new semi-dominant QTL for grain size and weight (GS2) in rice, which encodes the transcription factor OsGRF4 (GROWTH-REGULATING FACTOR 4) and is regulated by OsmiR396. We demonstrate that a 2 bp substitution mutation in GS2 perturbs OsmiR396-directed regulation of GS2, resulting in large and heavy grains and increased grain yield. Further results reveal that GS2 interacts with the transcription coactivitors OsGIF1/2/3, and overexpression of OsGIF1 increases grain size and weight. Thus, our findings define the regulatory mechanism of GS2, OsGIFs and OsmiR396 in grain size and weight control, suggesting this pathway could be used to increase yields in crops.