Reciprocal regulation of nuclear import of the yeast MutSalpha DNA mismatch repair proteins Msh2 and Msh6.
Reciprocal regulation of nuclear import of the yeast MutSalpha DNA mismatch repair proteins Msh2 and Msh6.
复制标题
酵母Mutsalpha DNA不匹配修复蛋白MSH2和MSH6的核进口的相互调节。
DOI：
10.1016/j.dnarep.2009.02.003
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发表时间：
2009-06-04
期刊：
影响因子：
3.8
通讯作者：
Gammie AE
中科院分区：
文献类型：
--
作者：
Hayes AP;Sevi LA;Feldt MC;Rose MD;Gammie AE
在真核生物中，DNA错配识别是由两种异源二聚体MutSα (Msh2/Msh6)和MutSβ (Msh2/Msh3)完成的，它们必须驻留在细胞核中才能发挥作用。两个推测的Msh2核定位序列（NLS）在Msh2背景下通过与绿色荧光蛋白（GFP）的融合和位点定向诱变进行了表征。一个NLS在GFP靶向分析中起作用，两个NLS都在Msh2中起冗余作用。我们检查了每个MutS单体在其伴侣存在和不存在的情况下的核定位。在缺乏Msh3和Msh6的细胞中，Msh2易位到细胞核；然而，缺乏Msh6的细胞显示细胞核Msh2水平显著降低。此外，在缺乏Msh6的情况下，Msh2的总蛋白水平显著降低，特别是在Msh2缺乏功能性NLS的情况下。Msh3在没有Msh2的情况下定位，而Msh6的定位依赖于Msh2表达功能性NLSs。总的来说，当另一个伴侣缺席时，Msh2和Msh6的核水平下降。这些数据提示了一种防止游离单体在细胞质中积累的稳定机制。
DNA mismatch recognition is performed in eukaryotes by two heterodimers known as MutSα (Msh2/Msh6) and MutSβ (Msh2/Msh3) that must reside in the nucleus to function. Two putative Msh2 nuclear localization sequences (NLS) were characterized by fusion to green fluorescent protein (GFP) and site-directed mutagenesis in the context of Msh2. One NLS functioned in GFP targeting assays and both acted redundantly within Msh2. We examined nuclear localization of each of the MutS monomers in the presence and absence of their partners. Msh2 translocated to the nucleus in cells lacking Msh3 and Msh6; however, cells lacking Msh6 showed significantly decreased levels of nuclear Msh2. Furthermore, the overall protein levels of Msh2 were significantly diminished in the absence of Msh6, particularly if Msh2 lacked a functional NLS. Msh3 localized in the absence of Msh2, but Msh6 localization depended on Msh2 expressing functional NLSs. Overall, the nuclear levels of Msh2 and Msh6 decline when the other partner is absent. The data suggest a stabilization mechanism to prevent free monomer accumulation in the cytoplasm.
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影响因子：
3.3
作者：
Fitch, PG;Gammie, AE;Rose, MD
通讯作者：
Rose, MD
影响因子：
64.8
作者：
Gavin, AC;Bösche, M;Superti-Furga, G
通讯作者：
Superti-Furga, G
影响因子：
56.9
作者：
DRUMMOND, JT;LI, GM;MODRICH, P
通讯作者：
MODRICH, P
DOI：
10.1073/pnas.95.21.12404
发表时间：
1998-10-13
影响因子：
11.1
作者：
Flores-Rozas, H;Kolodner, RD
通讯作者：
Kolodner, RD
影响因子：
9.2
作者：
Habraken, Y;Sung, P;Prakash, S
通讯作者：
Prakash, S