Prototheca cutis sp nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin
Prototheca cutis sp nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin
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DOI：
10.1099/ijs.0.016402-0
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发表时间：
2010-05-01
影响因子：
2.8
通讯作者：
Makimura, Koichi
中科院分区：
文献类型：
--
作者：
Satoh, Kazuo;Ooe, Kenji;Makimura, Koichi
从日本一家医院的一位原苔病患者的发炎皮肤中分离出一株新的致病性原苔属绿藻。通过组织病理学和培养检查在活检标本中检测和分离病原体。核18S rDNA基因和26S rDNA基因D1/D2结构域序列分析和化学分类研究表明，该菌株是一个新种，与wickerhamiprototheca wickerhamii和Auxenochlorella protothecoides有密切的系统发育关系。该菌株在28 ~ 30℃条件下生长良好，在37℃条件下生长缓慢弱，在40℃条件下无生长。该菌株在无维生素培养基中生长，以乙酸（pH 5.1）、l -阿拉伯糖和可溶性淀粉为碳源。提出了一种皮炎病原原鞘菌（Prototheca cutis sp. nov.）的分类描述(型菌株JCM 15793(T) =CBS 11262（T) =DSM 22084(T)）。
A strain of a novel pathogenic, achlorophyllic alga belonging to the genus Prototheca was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxononnic studies indicated that this strain represents a novel species with a close phylogenetic relationship to Prototheca wickerhamii and Auxenochlorella protothecoides. This strain grew well at 28-30 degrees C, showed slow and weak growth at 37 degrees C, and no growth at 40 degrees C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), L-arabinose and soluble starch as a carbon source. The taxonomic description of Prototheca cutis sp. nov. is proposed (type strain JCM 15793(T) =CBS 11262(T) =DSM 22084(T)) as a pathogen of dermatitis.