MITOCHONDRIAL-DNA SEQUENCES IN SINGLE HAIRS FROM A SOUTHERN AFRICAN POPULATION
MITOCHONDRIAL-DNA SEQUENCES IN SINGLE HAIRS FROM A SOUTHERN AFRICAN POPULATION
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DOI：
10.1073/pnas.86.23.9350
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
WILSON, AC
中科院分区：
文献类型：
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作者：
VIGILANT, L;PENNINGTON, R;WILSON, AC
从人的头发中提取基因组DNA，用酶法扩增线粒体DNA高变区，并直接测序。应用该方法研究了15个中国人的mtDNA序列变异。Kung population.一个系谱树将这些土著，讲科伊桑语的南部非洲人与其他68个人类和一只黑猩猩联系起来，在所有的非洲人中，它的分支最深！这一结果与人类mtDNA的非洲起源一致。15例在mtDNA控制区最可变部分具有相同序列的无关个体在人群中被发现！Kung，西部俾格米人和东部俾格米人，但在这些人群中没有明显的身份。非洲线粒体多样性的地理结构的这一证据和其他证据，以及对人类mtDNA碱基变化累积速率的了解，意味着女性血统在狩猎采集时代移动家园的平均速率可能低至每年13米。酶促扩增和直接测序技术应用于容易收集的高度稳定的生物材料，如头发，使得有可能以高分辨率检查几乎任何人群的许多代表。
Hypervariable parts of mitochondrial DNA (mtDNA) were amplified enzymatically and sequenced directly by using genomic DNA from single plucked human hairs. This method has been applied to study mtDNA sequence variation among 15 members of the ! Kung population. A genealogical tree relating these aboriginal, Khoisan-speaking southern Africans to 68 other humans and to one chimpanzee has the deepest branches occurring amongst the !Kung, a result consistent with an African origin of human mtDNA. Fifteen cases of unrelated individuals having identical sequences in the most variable parts of the mtDNA control region were foiund within populations of !Kung, Western Pygmies, and Eastern Pygmies, but no cases of identity were evident among these populations. This and other evidence of geographic structuring of the mitochondrial diversity in Africa, together with knowledge of the rate of accumulation of base changes in human mtDNA, implies that the average rate at which female lineages have moved their home bases during hunter-gatherer times could be as low as 13 meters per year. The technique of enzymatic amplification and direct sequencing applied to readily collected, highly stable biological materials such as hairs makes it possible to examine with high resolution many representatives of virtually any population.