Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast.
Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast.
复制标题
酵母 URA3 基因的结构和功能。
DOI：
10.1016/s0022-2836(83)80193-4
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发表时间：
1983
影响因子：
5.6
通讯作者：
Botstein,D
中科院分区：
文献类型：
--
作者：
Rose,M;Botstein,D
通过体外构建的ura3 - lacz基因融合体分析，研究了酿酒酵母ura3基因的表达。通过与orotidine-5 ' -phosphate decarboxylase （OMPdecarboxylase）编码序列在框架中融合合成的杂交β-半乳糖苷酶被发现在ura3编码序列仅保留11个核苷酸时正常受到调控，这表明所有的转录起始位点和调控位点都存在于ura3基因的开头。在另一个阅读框中开始一个短重叠编码序列的上游启动密码子也被发现在产生杂交β-半乳糖苷酶中具有活性。而这种β-半乳糖苷酶的合成几乎不受调节。核酸酶保护实验显示ura3mrna有多种存在。这与ura3蛋白和重叠肽是由不同长度的差异调节mrna翻译而来的观点是一致的。
Expression of theURA3gene ofSaccharomyces cerevisiaewas studied by analysis ofURA3-lacZgene fusions constructedin vitro. Synthesis of hybrid β-galactosidase by fusions in frame with the coding sequence for orotidine-5′-phosphate decarboxylase (OMPdecarboxylase) was found to be normally regulated even when only 11 nucleotides ofURA3coding sequence remained, indicating that all transcription initiation and regulatory sites are present at the beginning of theURA3gene. An upstream initiator codon that begins a short overlapping coding sequence in another reading frame was also found to be active in producing hybrid β-galactosidase. However this β-galactosidase synthesis showed little or no regulation. Nuclease protection experiments revealed numerous species ofURA3mRNA. The regulation of these is consistent with the idea that theURA3protein and the overlapping peptide are translated from differentially regulated mRNAs of different lengths.