Platelet-derived growth factor expression in mesangial proliferative glomerulonephritis.
Platelet-derived growth factor expression in mesangial proliferative glomerulonephritis.
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发表时间：
1991-08
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通讯作者：
L. Gesualdo;M. Pinzani;J. Floriano;M. Hassan;N. Nagy;F. Schena;S. Emancipator;H. Abboud
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作者：
L. Gesualdo;M. Pinzani;J. Floriano;M. Hassan;N. Nagy;F. Schena;S. Emancipator;H. Abboud
系膜细胞增生和系膜基质扩张是增生性肾小球疾病的常见组织学特征，是肾衰竭的常见病因。肾小球系膜细胞的增殖是对血小板衍生生长因子（PDGF）的反应，这些细胞释放PDGF并表达PDGF A和B链mrna。然而，迄今为止，所有关于PDGF与肾小球结构和功能潜在变化的研究都是在体外进行的。为了探讨PDGF在增生性肾小球肾炎中的作用，我们研究了两种不同肾小球损伤组织学模式（系膜增生为主或系膜基质扩张为主）的IgA肾病动物模型中PDGF的体内表达。免疫组织化学技术和溶液杂交技术分别证实PDGF和PDGF b链mRNA在患病小鼠全肾中的表达增加。免疫组化结果显示，PDGF主要位于肾小球系膜区，间质中PDGF的表达程度较低。PDGF表达的增加与高细胞化程度和疾病的临床特征相关。此外，PDGF在某些形式的人肾小球肾炎中表达增加，其特征是系膜增生。这些发现提示PDGF可能是增生性肾小球肾炎中系膜细胞增殖的主要因素。
Proliferation of mesangial cells and expansion of mesangial matrix are common histologic features of proliferative glomerular disease, a frequent cause of renal failure. Proliferation of glomerular mesangial cells occurs in response to platelet-derived growth factor (PDGF), and these cells release PDGF and express PDGF A and B chain mRNAs. However, all studies relating PDGF to potential changes in glomerular structure and function to date have been performed in vitro. To explore the role of PDGF in proliferative glomerulonephritides, we studied the expression of PDGF in vivo in two animal models of IgA nephropathy with different histologic patterns of glomerular injury: either predominant mesangial proliferation or expansion of mesangial matrix. Increased expression of PDGF and PDGF B-chain mRNA in whole kidneys from diseased mice was demonstrated by immunohistochemical techniques and by solution hybridization assay, respectively. Immunohistochemically, PDGF was localized primarily within the mesangial area of glomeruli and to a much lower extent in interstitium. The increased PDGF expression correlated with the degree of hypercellularity and clinical features of the disease. In addition, PDGF expression was increased in some forms of human glomerulonephritis, characterized by mesangial proliferation. These findings suggest that PDGF may be a major contributor to mesangial cell proliferation seen in proliferative glomerulonephritides.