Does the host's developmental stage affect host preference following an ovipositioning experience in Heterospilus prosopidis?
Does the host's developmental stage affect host preference following an ovipositioning experience in Heterospilus prosopidis?
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DOI：
10.1111/eea.12490
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发表时间：
2016-10-01
影响因子：
1.9
通讯作者：
Shimada, Masakazu
中科院分区：
文献类型：
--
作者：
Sasakawa, Koji;Abe, Masato S.;Shimada, Masakazu
许多寄生蜂表现出产卵学习，即雌性将与寄主相关的线索与产卵联系起来，并在随后寻找寄主时使用这些线索。这是受各种因素的影响，这些因素尚未完全阐明。本文研究了寄主发育阶段对利用幼虫和蛹的寄生蜂产卵学习的影响，以Bruchinae甲虫(鞘翅目：叶甲科)的体外寄生蜂Heterspilus prosopidis Viereck为模型。首先，我们研究了在幼虫和蛹上产卵对寄主物种偏好的诱导是否存在差异。用两种同样喜好的寄主--中国绿豆象Callsobruchus chinensis(L.)和斑纹豆象Callosobruchus maculatus(Fabricius)-以及在这两个寄主物种上的产卵经历而受到限制的黄蜂。我们发现，在有蛹寄主的黄蜂中诱导寄主物种偏好，而在有幼虫寄主的黄蜂中没有诱导寄主种类偏爱。接下来，我们研究了诱导偏爱是否与寄主阶段有关。以以蛹为寄主的黄蜂和幼虫为选择寄主的产卵试验表明，在寻找幼虫寄主时不使用学习线索。在幼虫寄主条件下的黄蜂中也得到了类似的结果。我们比较了幼虫和幼虫的产卵行为，但没有发现可能导致观察到的寄主偏好诱导差异的行为差异。我们的结果表明，幼虫和幼虫在产卵后对寄主的选择性有不同的影响，尽管具体机制尚不清楚。这些发现值得注意，因为在同时利用幼虫和幼虫寄主的寄生蜂中，寄主发育阶段作为影响产卵学习的因素几乎没有引起注意。
Many parasitoid wasps exhibit 'oviposition learning,' whereby females associate host-related cues with oviposition and use them in subsequent searches for hosts. This is affected by various factors that have yet to be fully elucidated. We examined the effects of host developmental stage on oviposition learning in parasitoid wasps that utilize both larval and pupal hosts using Heterospilus prosopidis Viereck (Hymenoptera: Braconidae), an ectoparasitoid of Bruchinae beetles (Coleoptera: Chrysomelidae), as amodel. First, we examined whether the induction of host species preference differs between oviposition on larval and pupal hosts. Oviposition choice experiments were performed for both larval and pupal hosts using two equally preferred host species - Callosobruchus chinensis (L.) and Callosobruchus maculatus (Fabricius) - and wasps that were conditioned by an oviposition experience on either of the two host species. We found an induction of host species preference in wasps conditioned with pupal hosts but not in wasps conditioned with larval hosts. Next, we examined whether the induced preference was host-stage-specific. Oviposition experiments with pupal-host-conditioned wasps and larval hosts as choice hosts indicated that learned cues were not used when searching for larval hosts. Similar results were obtained for larval-host-conditioned wasps. We compared oviposition behavior on larval and pupal hosts but found no behavioral differences that could contribute to the observed difference in host preference induction. Our results indicate that larval and pupal hosts have different effects on host preference following an oviposition experience in H. prosopidis, although the detailed mechanisms remain unclear. These findings are notable because in parasitoid wasps that utilize both larval and pupal hosts, host developmental stages have attracted little attention as factors affecting oviposition learning.